骆驼刺高效离体植株再生体系的建立

Establishment of a high efficient regeneration system in Alhagi pseudalhagi

通过对骆驼刺 (Alhagi pseudalhagi Desv)不同外植体来源、不同培养基及激素配比的比较 ,建立骆驼刺组织培养高效再生植株实验体系。表明骆驼刺下胚轴切段在含 1 .5～ 2 .0 mg/L2 ,4- D和 0 .5～ 1 .0 mg/L 6- BA的 MS培养基中 1 0 0 %诱导愈伤组织 ,在含 1 .5mg/L 6- BA和 1 .0 mg/LNAA的 MS培养基上愈伤组织分化成苗 ,转至含 2 .0 mg/L IBA和 0 .2 mg/L NAA的 MS培养基上成根得到完整再生植株。组织学观察表明植株再生主要为器官发生途径 ,染色体稳定遗传。

After sterilizing, seeds of A.pseudalhagi were planted on Ms medium without phytohormone. Hypocotyl segments of 5cm in length were excised and cultured on Ms medium containing 1.5～2.0mg/L 2,4 D and 0.5～1.0 mg/L 6 BA to induce calluses. The calluses were subcultured on the same medium. Shoot differentiated from callus on the Ms medium supplementing 1.5 mg/L 6 BA and 1.0 mg/L NAA. The shoots were transferred on the MS medium with 2.0 mg/L IBA and 0.2 mg/L NAA, roots were induced. The differentiation frequencies of calluses subcultured for two years were still 100% and the cytological observation revealed chromosomes kept genetic stability in the resulting materials. The regeneration of A.pseudalhagi were mainly organogenesis.