7岁以下儿童细菌感染耐药分析

Analysis of antibiotic resistance of bacteria in children under seven years

目的观察大连地区5家医院(大连医科大学附属一院、大连医科大学附属二院、大连儿童医院、大连市第六人民医院、大连开发区医院)2012年7岁以下儿童感染病原菌分布及其耐药情况。方法临床分离菌株,采用细菌分离鉴定方法(API、VITEK、Microsean系统)进行目标细菌的鉴定,药物敏感性试验用K-B纸片扩散法测定药物的敏感性。结果共收集细菌1 235株,其中革兰阴性菌725株占58.7%,革兰阳性菌510株占41.3%;分离细菌前5位依次为大肠埃希菌占14.9%、凝固酶阴性葡萄球菌占13.6%、金黄色葡萄球菌占11.7%、肺炎克雷伯菌占10.8%、铜绿假单胞菌占7.4%。结论 7岁以下儿童感染致病菌对抗生素均存在不同程度耐药情况,期望在临床治疗感染时有所帮助。

Objective To observe pathogens distribution and antibiotics resistance infected by children under seven years in Dalian. Methods The strains are clinically isolated, and the target bacteria is identified through bacteria separated identification method（ API, VITEK and Microsean system） , Kirby-Bauer disc diffusion method was used to test the drug sensitivity. Results 1 235 strains of bacteria were collected, Gram negative bacteria being 725 strains（58.7% ）, Gram positive bacteria being 510 strains（41, 3% ） ; The 5 top of isolated bacterias were Escherichia coli（ 14. 9% ） , coagulase negative Staphylococcus （ 13.6% ） , Staphylococcus aureus （ 11.7% ）, Klebsiella pneumouiae（ 10. 8% ） and Pseudomonas aeruginosa（7.4% ）, respectively. Conclusion In children under seven years, pathogenic bacteria exists different degrees of resistance to antibiotics, and it maybe helpful in clinical treatment.