蒙成药哈日嘎布日-10味片中胡椒碱含量测定

Content Determination of Piperine in Monglia Medicine Harigaburi-10 Tablets

目的：建立蒙成药哈日嘎布日－10味片中胡椒碱含量测定方法。方法：采用高效液相色谱法测定，Agilent Eclipse XDB C18色谱柱（4．60mm×250mm，5μm），流动相甲醇－水（70：30），检测波长343nm，流速1．0mL·min^-1。结果：胡椒碱在0．0107～0．0856μg呈现良好的线性关系（r=0．9999），平均回收率101．8％（n=6），RSD1．8％。结论：该方法具有简便、快速、准确、重复性好，可用来控制蒙成药哈日嘎布日-10味片的质量。

Objective ： To establish a feasible method for the content determination of piperine in Monglia Medicine Harigaburi-10 tablets by HPLC. Method： An HPLC method was adopted with column Agilent Eclipse XDB C18 （4.6 mm × 250 mm, 5 μm）. The mobile phase was consisted of methanol-water （70： 30）. The flow velocity was 1.0 mL .min-1 The detection wavelength was at set at 343 nm. Result： The calibration curve showed a good linearity within the range of 0. 010 7-0. 085 6 μg. The average recovery was 101.8% （n =6） , RSD 1.8%. Conclusion： This method is convenient, rapid and reliable for quality control of this preparation.