芹菜素抗肺癌SPC-A1细胞增殖和诱导凋亡作用

Effect of Apigenin on Proliferation and Apoptosis in Human NSCLC SPC-A1 Cell Line

目的：探讨芹菜素对人非小细胞肺癌SPC—Al细胞增殖和凋亡的影响。方法：体外常规培养人肺癌细胞株SPC—Al细胞，用5～80μmol·L^-1不同浓度芹菜素作用SPC—Al细胞，MTT法检测细胞增殖抑制率；Hoechst33258染色法观察细胞凋亡的形态学变化；流式细胞术FITC—AnnexinV／碘化丙啶（PI）双标记染色法检测细胞凋亡率。结果：芹菜素对SPC—Al细胞增殖的抑制作用呈时间和浓度依赖性，芹菜素干预24，48，72h的IC50值分别为319．02，37．23，18．59μmol·L^-1；Hoechst33258染色后倒置荧光显微镜下观察可见芹菜素给药后出现细胞数逐渐减少，凋亡细胞核固缩，染色质凝集，着色深而呈亮蓝色；流式细胞术检测结果显示芹菜素可诱导SPC—Al细胞出现不同程度的凋亡。结论：芹菜素能抑制人非小细胞肺癌SPC—Al细胞的增殖和诱导其凋亡。

Objective： To investigate the effect of apigenin on the growth inhibition and apoptosis in human lung cancer cell line SPC-Al. Method： Lung cancer cell line SPC-Al were cultured in vitro routinely with different concentration （5-80 μmol .L^-1 ）. Cell proliferation was detected by MTT. Hoechst 33258 was used to detect the morphological change of apoptotic cells. Stained by Annexin V and propidium iodide （PI） , the cells were observed by flow cytometry for apoptosis. Result： The proliferation of human lung cancer cell line SPC-Al could be inhibited and apoptosis could be induced by apigenin. The inhibition rate and apoptosis rate were different with the different concentrations. The 50% inhibitory concentration （ICs0） at the 24, 48, 72 h was 319. 02, 37. 23, 18. 59 μmol .L ^-1 respectively. Apoptotic body could be observed by Hoechst 33258 dying. Flow cytometry showed that apigenin could induce SPC-Al cell apoptosis distinctly at different levels. Conclusion： Apigenin could inhibit the proliferation and induce apoptosis of human non-small cell lung cancer SPC-Al.