鳖甲水提物和醇提物对单胺氧化酶活性影响的研究

Effect of Carapax trionycis' Water Extract and Ethanol Extract on MAO Activity

目的:评价鳖甲水提物和醇提物总氨基酸及寡肽类成分含量及两种提取物对单胺氧化酶(MAO)活性影响的比较研究,考察两种化学成分与药效的关系,探讨鳖甲提取工艺及有效组分。方法:分别采用水提和醇提制备鳖甲药材的提取物,分别以谷氨酸和鳖甲七肽为标准物质建立总氨基酸和寡肽类含量测定标准曲线,测定鳖甲水提物和醇提物总氨基酸和寡肽类含量,并按照单胺氧化酶试剂盒说明书测定两种提取物对体外MAO活性作用。结果:水提法和醇提法的浸膏得率分别为20.6%和5.4%;鳖甲水提物和醇提物中寡肽类含量分别(48.13±3.27)%和(20.17±2.49)%;总氨基酸含量分别为(23.68±1.76)%和(55.88±2.41)%;水提物和醇提物均具有抑制MAO活性作用,MAO抑制率分别为(65.12±1.34)%和(50.95±1.13)%。结论:鳖甲水提物浸膏得率及寡肽含量均大于醇提法;总氨基酸含量则是醇提物高于水提物。二者均有抑制MAO活性效果,且水提物的MAO抑制效果略优于醇提物;提示鳖甲中寡肽类成分和总氨基酸类成分都具有抑制MAO活性作用,且水提物效果更佳,因此鳖甲以水提为佳,推测寡肽类成分和氨基酸类成分可能均为其有效物质。

Objective： To evaluate effect of the Carapax trionycis＇ total amino acids and oligo-peptide＇ content of the aqueous extract and alcohol extract on monoamine oxidase （MAO） activity. Method： C. trionycis aqueous extract and alcohol extract were prepared, then glutamic acid and C trionycis oligo-peptide were used to establish standard material content respectively to calculate aqueous extract and alcohol extract total amino acid and oligo-peptide content, and observe the effect on MAO in vitro. Result： The extracted yield of the aqueous extract and alcohol extract was 20.6% and 5.4% , respectively; the oligo-peptide content of the aqueous extract and alcohol extract was （48.13 ±3.72）% and （20. 17 ± 2.49）% respectively; the total amino acid content of the aqueous extract and alcohol extract was （23.68 ± 1.76）% and （55.88 -± 2.41 ）% respectively; the aqueous extract and alcohol extract have inhibitory effect on MAO and the inhibition rate was （65. 12 ± 1.34） % and （50.95 ± 1. 13）% respectively. Conclusion： After the water was extracted, the extract yield is better than that of the alcohol extract; the aqueous extract and alcohol extract have inhibitory effect on MAO activity, and the effect of the aqueous extract is slightly stronger than that of the alcohol extract; the aqueous extract oligo-peptide content was higher than that of alcohol extract; alcohol extract total amino acid content is higher than that of the aqueous extract. It is better to extract the C using water, and water soluble constituents and alcohol soluble components in C have inhibitory activity on MAO and they are effective chemical constituents