长筒石蒜叶片薄层培养诱导愈伤组织发生的研究

Study on Callus Induction of the Leaf Blades of Lycoris longituba Using Thin Cell Layer Culture

以切成2mm左右的长筒石蒜幼嫩叶片薄片作为外植体,研究了不同激素组合以及不同接种方式对其愈伤组织诱导的影响,以期获得长筒石蒜叶片愈伤诱导的最佳条件。结果表明:在1/2MS+NAA 0.5mg/L+TDZ 2.0mg/L+蔗糖30g/L+土豆粉3g/L培养基中,愈伤诱导率最高可达90.0%,TDZ诱导愈伤效果明显优于2,4-D;采用叶片垂直插入培养基的接种方式,培养30d后获得愈伤诱导率最高为83.3%。

The young leaves of Lycoris longituba that were cut into 2 rnm slices as explants, which cultured in vitro on several mediums supplemented with diverse concentrations of plant growth regulator and different inoculation method in order to find the best conditions of callus induction. The results showed that the highest induction rate 90. 0% was obtained from the mediums which consist of 1/2MS＋NAA 0. 5 mg/L＋TDZ 2. 0 mg/L＋ sucrose 30 g/L＋dried potato powder 3 g/L. Callus induction effect of TDZ was obviously better than that of 2,4-D; adopts blades vertically inserted into the culture medium of inoculation method, cultivation of 30 days could obtain the highest callus induction rate of 83. 3 %.