U74006F对缺血前高血糖大鼠局部脑缺血再灌注损伤保护作用的研究

The protective effects of U74006F on focal cerebral ischemic reperfusive injury in preischemic hyperglycemic rats

目的探讨Ｕ７４００６Ｆ对缺血前高血糖大鼠局部脑缺血再灌注损伤保护作用。方法线栓法建立脑缺血再灌注模型。大鼠缺血前３０分钟注射５０％葡萄糖，使之处于高血糖状态，再灌注前静注Ｕ７４００６Ｆ（６ｍｇ／ｋｇ）。另设枸橼酸盐组与对照组。然后比较３组大鼠梗死面积、神经功能缺损症状和脑组织病理改变。结果Ｕ７４００６Ｆ组梗死面积较枸橼酸盐组平均缩小６４％（Ｐ＜０．０１），神经功能评分显著降低（Ｐ＜０．０５），病理改变明显减轻。对照组损伤程度也显著轻于枸橼酸盐组（Ｐ＜０．０５）。结论 高血糖可加重脑缺血再灌注损伤。Ｕ７４００６Ｆ对高血糖状态下大鼠缺血再灌注损伤有明显保护作用。

Objective To explore the protective effect of U74006F on focal cerebral ischemic reperfusive injury in preischemic hyperglycemic rats. Methods Using thread embolism method to make the model of cerebral ischemic reperfusion in rats. 50% glucose was injected 30 min prior to ischemia,and U74006F (6 mg/kg) was injected before reperfusion. In addition,citrate acid group and control group were set. Then compared with infarct size, neurological dysfunction and the pathological change among the rats in three groups. Results The infarct size in U74006F group was narrower than the citrate acid group 64% (P＜0.01) and neurological function score reduced significantly (P<0.05), the pathological change lightened significantly. The injury degree was more mild in the control group than in the citrate acid one (P<0.05). Conclusions Preischemic hyperglycemia could augment the focal cerebral ischemic reperfusive injury. U74006F had obvious protective effect on focal ischemic reperfusive injury in preischemic hyperglycemic rats.