转基因玉米T25基体标准物质协同实验研究

Characterizing on Genetically Modified T25 Maize Line Matrix Reference Material

对转基因玉米125转化体特异性荧光定量PCR方法的关键参数进行了方法验证，选择了7家实验室采用经过验证的方法对3个水平的转基因玉米125基体标准物质进行协同实验研究。通过比较分析各参与实验室的实验条件、标准曲线的参数，进一步证明该方法室间重复性好。经统计学分析各参与实验室的数据等精度，因此计算各实验室的总平均值做为标准物质的标准值。3个水平的基体标准物质的标准值和扩展不确定度（k=2）分另Ⅱ为1．17％±0．22％、2．17％±0．38％和9．81％±2．30％。

To validate the key parameters of the real time PCR method for GM maize T25, 7 different laboratories are chosen to assign the reference values of three different GM content matrix reference materials by using the same method. Although different types of instruments are used by different labs, the standard curve generated by each lab is comparable which indicated that this method shows a good reproductablity. After statistically analyzing the data gained from seven labs, the total means are assigned to be the reference values. The reference values and expanded uncertainties （k = 2） for three matrix GM reference material are 1.17% ±0. 22% ,2. 17% ±0. 38% and 9. 81% ±2. 30%.