摘要
采用单因素实验和正交试验,优化了鼠源α-葡萄糖苷酶抑制剂体外微量筛选模型,并应用该模型研究泽泻正丁醇萃取部分(AOB)在体外对小鼠α-葡萄糖苷酶的抑制活性。结果显示,AOB体外具有较强的小鼠小肠α-葡萄糖苷酶抑制活性,IC50为126μg/mL。灌胃10 d后,AOB能有效地抑制小鼠体内α-葡萄糖苷酶活性,其活性明显低于生理盐水组。AOB是潜在的高效α-葡萄糖苷酶抑制剂。
Slight modifications of microplate-based screening model were carried out with single factor and orthogonal ex- periments. The inhibitory effect of n-butanol fraction of Alisma orientailis （AOB） on mice intestinal α-glucosidase was assayed by this model. The results showed that AOB significantly inhibited mice intestinalα-glucosidase activity with a dose dependent manner with ICso value of 126 μg/mL in vitro and suppressed intestinal α-glucosidase activities in nor- real mice compared with control group after orally administered for 10 days. AOB has the potential to inhibit activities of mice α-glucosidase.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2013年第12期1644-1648,共5页
Natural Product Research and Development
基金
国家自然科学基金项目(31060216)
