垂丝海棠抗氧化活性的研究

Study on antioxidant activity of Malus halliana Koehne

采用清除二苯代苦味酰基(DPPH)自由基、清除[2,2'-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐](ABTS)自由基及铁离子还原/抗氧化能力(FRAP)测定法对不同采摘月份垂丝海棠花和叶进行体外抗氧化活性评价,并与阳性对照二丁基羟基甲苯(BHT)进行比较。结果表明:在垂丝海棠12个提取物中,3月叶正丁醇部位清除DPPH自由基及还原Fe3+(IC50=35.80μg/mL,TEAC=869.73μmol/g)最强,弱于阳性对照BHT(IC50=23.00μg/mL,TEAC=1532.70μmol/g);3月叶乙酸乙酯提取物清除ABTS自由基(IC50=3.50μg/mL)能力最强,弱于阳性对照BHT(IC50=2.30μg/mL)。实验表明垂丝海棠3月采集的叶抗氧化活性总体好于7月叶,并且叶的活性强于花。

To study the antioxidant activity of flowers and leaves of Malus halliarm Koehne harvested in different months, 1 -diphenyl-2 -picrylhydrazyl （DPPH） radical scavenging, [ 2,2＇-azino-bis （ 3-ethylbenzothiazoline ） -6 -sulphonic acid ] diamonium salt （ABTS） radical scavenging and ferric reducing antioxidant power （FRAP） assay were applied with BHT as positive control. Among twelve extracts of the leaves and flowers of M. halliana,the results showed that the n-butanol extract from March leaves had the best DPPH radical scavenging activity （IC5o = 35.80 μg/mL） and ferric reducing an- tioxidant power （TEAC = 869.73 μmol/g）, which were still weaker than that of the positive control BHT （ IC50 = 23.00μg/mL and TEAC = 1532.70μmol/g respectively）. The ethyl acetate extract of leaves collected in March exhibited the highest free radical scavenging activity （IC50 =3.50 μg/mL） ,but it was still inferior to positive control BHT （IC50 = 2.30 txg/mL）. It demonstrated that antioxidant activity of M. ha/I/arm was weak. The extract of leaves harvested in March had better activities than that of leaves harvested in July, and the antioxidant activities of extract of leaves were higher than that of flowers.