分子信标的构建及其应用研究进展

Construction of Molecular Beacons and Their Research Progress of Application

分子信标(molecular beacon,MB)是一种寡聚核苷酸荧光探针,其具有灵敏度高、特异性强、操作简单以及不必与未反应的探针分离即可实时检测等优点,在分子生物学和基因组学及分子医学等领域具有十分重要的应用价值。本文介绍了近年来出现的各种新型分子信标的结构及工作原理,概述了分子信标技术在生命科学领域中的应用,展望了分子信标技术的发展趋势。

Molecular beacon is an oligonucleotide probe of fluorescence. Molecular beacons are playing a very important role in molecular biology, genetics and molecular medicine because they have been shown to have very high selectivity, high sensitivity, and real-time detection capabilities. The structures and working mechanisms of a variety of novel molecular beacons have been outlined here. We briefly summarize the application of molecular beacon technology in the field of life sciences, and propose the prospect this technology.