摘要
目的:建立测定人血浆中盐酸右美托咪定浓度的方法。方法:血浆样品用乙酸乙酯-二氯甲烷(4:1)萃取浓缩后进样。采用液一质联用(LC-MS/MS)法进样测定,以替米沙坦为内标,色谱柱为Agilent Eclipse Plus C18,流动相为甲醇-1%甲酸水(75:25,V/V);采用电喷雾离子源(ESI),以多反应离子检测(MKM)方式进行检测。右美托咪定和内标检测离子对的m/z分别为201.1/g5.1、512.2/276.1。结果:右美托咪定血药浓度在20~6000ng/L范围内线性关系良好(r=0.9951),最低定量限为20ng/L,提取回收率为82.20%~96.37%,日内、日间RSD均在11%之内。结论:本方法具有快速、灵敏、重现性好等特点,可用于临床上盐酸右美托咪定血药浓度监测及药动学研究。
OBJECTIVE: To establish a method for the determination of dexrnedetomidine hydrochloride in human plasma. METHODS: Dexmedetomidine was extracted with acetic ether-dichloromethane (4:1) and LC-MS/MS was adopted using telmisartan as internal standard. The separation was performed on Agilent Eclipse Plus C18 column with mobile phase consisted of methanol-1% formic acid (75:25, V/V). ESI. source was applied. Dexmedetomidine and telmisartan (IS) were detected on multiple reaction monitoring (MRM) mode by the transitions from the precursor to the production (m/z 201.1/95.1,512.2/276.1). RESULTS: The linear range of dexmedetomidine were 20-6 000 ng/L (r=0.995 1), and the lowest quantification limit was 20 ng/L. The relative recoveries were 82.20%-96.37%, and the RSDs of intra-day and inter-day were less than 11%. CONCLUSIONS: The method is proved to be rapid, sensitive and reproducible for the determination of dexmedetomidine in human plasma and pharmacokinetic study.
出处
《中国药房》
CAS
CSCD
2014年第2期126-128,共3页
China Pharmacy
基金
全军医学科研"十二五"面上项目(No.CWS11J269)
广东省广州市科技攻关计划项目(No.201300000176)
关键词
盐酸右美托咪定
液
质联用法
血药浓度
Dexmedetomidine hydrochloride
LC-MS/MS
Plasma concentration