摘要
将从菊芋中克隆到的蔗糖:蔗糖-1-果糖基转移酶基因(即1-SST基因),与根部特异性表达启动子pPST2a组合,通过农杆菌介导法获得转pPST2a:1-SST基因甘蔗植株,通过分子检测共得到15个阳性转基因株系。将前期工作得到的转rbcs:1-SST基因甘蔗5个株系与转pPST2a:1-SST基因甘蔗进行抗旱性比较。结果发现,转pPST2a:1-SST基因甘蔗的抗旱性高于转rbcs:1-SST基因甘蔗。与启动子rbcs相比,启动子pPST2a能够更大程度上增强转1-SST基因甘蔗植株的抗旱性。
Fructan is a nonreducing polysaccharides of glucose that functions as a protectant in the stabilization of biological structures and enhances the stress tolerance under abiotic stresses in organisms. The sucrose:sucrse 1- fructosyltransferase (1-SST) gene from Jerusalem artichoke combined with root specific promoter pPST2a was transferred into sugarcane(Saccharumofftcinaram L.) using Agrobatorium-mediated method. Results indicated that 15 pPST2a:I-SST transgenic lines were successfully obtained according to molecular detection. Compared the drought resistance of the transgenic sugarcane between the one with pPST2a:1-SST lines obtained in the study and the other one with rbcs:1-SST obtained in previous research. The resuults showed that drought resistance of the transgenic sugarcane with pPST2a:1-SST lines was better than the one with rbcs:l-SST. And it revealed that promoter pPST2a could make drought resistance of transgenic sugarcane even stronger than the promoter rbcs.
出处
《热带作物学报》
CSCD
北大核心
2014年第1期1-6,共6页
Chinese Journal of Tropical Crops
基金
863重点项目(No.SS2013AA100604)
中央级公益性科研院所基本科研业务费专项(No.1630052012013
No.ITBB120501)
关键词
果聚糖
1-SST
转基因甘蔗
抗旱性比较
Fructan
1-SST
Transgenic sugarcane
Comparison of drought resistance