海南黄灯笼辣椒小叶病病原物的分子鉴定

Molecular Identification of the Pathogen of Capsicum chinense Jacquin Little-leaf Disease in Hainan

采用分子生物学技术对田间疑似植原体侵染引起的小叶症状的黄灯笼辣椒样品进行检测,以明确其病原分类地位。利用植原体通用引物对R16mF2/R16mR1和R16F2n/R16R2,对上述黄灯笼辣椒叶片DNA进行巢式PCR扩增。结果表明:获得约1.2 kb的特异片段,经序列测定、Blast同源性检索及进化树和iPhyClassifier分析,获得该特异片段长为1 248 bp,与植原体16S rⅡ组中的花生丛枝植原体(L33765)同源性最高,达99%,聚类在同一个进化枝上,相似性系数为1.00,归属于16S rII-A亚组。暂将其命名为黄灯笼辣椒小叶植原体(Capsicum chinense Jacquin little-leaf disease phytoplasma,CcJLL)。

In the paper, to ascertain taxonomic status of pathogen, Capsicum chinense Jacquin samples with little leaf symptom which were suggestive of phytoplasma infection, were detected by molecular biology. Primers R16mF2/R16mR1 and R16F2n/R16R2 were used to amplify the DNA samples from symptoms above by nested PCR. The PCR products were sequenced and Blast searching in GenBank. The phylogenetic tree and iPhyClassifier analysis of the 16S rDNA sequences showed that the sequence shared the identity of 99% with that from Peanut witches＇-broom（L33765）, and clustered in the same clade, with similarity coefficient of 1.00. The phytoplasma belonged to 16S rlI-A subgroup, was tentatively named as Capsicum chinense Jacquin little-leaf disease phytoplasma, CcJLL.