大黄对创伤性休克大鼠二胺氧化酶和内毒素的影响

Effects of Rhubarb Powder on Diamine Oxidase and Endotoxin of Traumatic Shock Rats

目的:观察创伤性休克大鼠二胺氧化酶(DAO)和内毒素变化及大黄的对其影响作用。方法:健康SD大鼠72只,分为正常对照组(8只)、创伤性休克组(32只)以及大黄干预组(32只)。观察二胺氧化酶和内毒素随时间的变化趋势、回肠形态学及大黄对上述指标的影响。结果:与对照组相比,创伤性休克和大黄干预组DAO活性在伤后2h均显著升高(P<0.01),伤后48h达高峰,伤后72h降低,但仍高于对照组(P<0.01)。而两组内毒素水平则在伤后2h均显著升高(P<0.01),伤后24h达高峰,伤后48h和72h有所降低,但仍高于对照组(P<0.01)。而休克组和大黄干预组之间比较发现,DAO活性和内毒素水平在伤后2h差异无显著性(P>0.05),而24h、48h和72h休克组则显著高于大黄干预组(P<0.01)。休克各组中,可见肠黏膜绒毛水肿,稀疏、片状坏死、脱落,中性粒细胞浸润及淋巴滤泡增生等病理改变,大黄干预组肠黏膜在伤后各时间点也可见上述变化,但较上述各组明显改善。结论创伤性休克早期即有肠屏障功能的严重受损,动态监测DAO和内毒素对了解创伤性休克大鼠肠屏障功能的改变有重要意义,大黄对创伤性休克大鼠具有胃肠道保护作用,从而有降低DAO和内毒素的作用。

Objective ： To observe the change of diamine oxidase （DAO） and endotoxin concentration and the effects of the rhubarb powder on them in the traumatic shock rats. Methods：72 healthy SD rats were divided into normal control group （ 8）, traumatic shock group （32） and rhubarb intervention group （32）. The change of the diamine oxidase and en- dotoxin concentration,ileum morphology and the influence of rhubarb in those rats were observed. Results：The diamine oxidase concentration in the traumatic shock group and the rhubarb intervention group had been significantly increased in 2 hour after injury （P 〈 0.01 ） ,reached to the concentration peak in 48 hour after injury and reduced in 72 hour after in- jury, but it was still higher than that of the normal control group. There was no significant difference between the shock group and the rhubarb intervention group in 2 hour after injury,but the shock group was significantly higher than the rhu- barb intervention group in 24 hour,d8 hour and 72 hour after injury. Compared with the normal control group, the endo- toxin concentration in the traumatic shock group and the rhubarb intervention group had been significantly increased in 2 hour after injury （P 〈 0.01 ） ,reached to the concentration peak in 24 hour after injury and reduced in 48 hour and 72 hour after injury, but still higher than that of the normal control group. It was shown in the results that the change of endo- toxin concentration was the same as the diamine oxidase （DAO） concentration. Pathological changes were visible in the traumatic shock group, such as intestinal mucosa edema of villi, sparse flake off necrosis, neutrophils infiltrating and lymph follicle proliferation and so on. The same changes had occurred in the rhubarb intervention group at the same time after injury, but these had been obviously improved. Conclusions：The intestinal barrier function was seriously damaged in the early stage of traumatic shock rats. It has important significance for the change of intestinal barrier function in the traumatic shock rats by dynamic monitoring DAO and endotoxin concentration. The rhubarb powder has the effect of protecting the gastrointestinal tract to reduce DAO and endotoxin concentration in traumatic shock rats.