高效液相色谱法同时测定桑菊感冒片中绿原酸、木犀草苷和3,5-O-二咖啡酰基奎宁酸含量

Content Determinations of Chlorogenic Acid,Luteolin Glycosides and 3,5-O-Dicaffeoylquinic Acid in Sangju Ganmao Tablets with HPLC

目的：建立高效液相色谱法同时测定桑菊感冒片中绿原酸、木犀草苷和3,5-O-二咖啡酰基奎宁酸含量,以提高原质量标准。方法：样品用50%甲醇溶液提取,采用Phenomenex C18色谱柱（5μm,4.6mm×250mm）分离;流动相为0.1%磷酸-乙腈,梯度洗脱;流速为1.0mL.min-1;检测波长为348nm。结果：绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸分别在2.8128～140.6400μg.mL-1、0.9580～47.9023μg.mL-1、0.9879～49.3944μg.mL-1范围具有良好的线性;加标回收率分别为99.1%、100.9%、100.1%;RSD分别为1.5%、1.8%、1.8%（n=9）。结论：该方法简单易行,准确性和重复性好,可用于桑菊感冒片的质量控制。

Objective： To establish a method for the content determinations of chlorogenic acid,luteolin glycosides and 3,5 - O - dieaffeoylquinic acid in Sangju Ganmao Tablets. Method： The sample was extracted by 50% methanol and sep- arated on a Phenomenex ODS column（5 ixm,4.6 mm ~250 mm）. The mobile phases were 0. 1% phosphoric acid and aeetonitrile and gradient elution. The flow rate was 1.0 mL . rain-1 while the detective wavelength was set at 348 nm. Result ： The linear ranges of chlorogenic acid, luteolin glycosides and 3,5 - O - dicaffeoylquinic acid were respectively in 2. 8128 - 140.6400μg.mL-1 ,0.9580 -47. 9023μg.mL-1 ,0.9879 -49. 3944 μg.mL-1 while the average recoveries was 99.1% , 100.9% ,100.1% with the RSD 1.5% , 1.8% , 1.8% （ n = 9）. Conclusion ： This method is simple, rapid and with good repeatability, so it could be used in quality control of Sangju Ganmao Tablets.