摘要
目的 :从动物组织中提取纯化核心蛋白聚糖 (Decorin) ,检测其活性。方法 :以大鼠肌肉为原料 ,采用匀浆、透析、层析等步骤分离、纯化Decorin ,经SDS PAGE检测其分子量和纯度 ,并经细胞株A5 49检测生物学活性结果 :Decorin在SDS PAGE显示分子量约为 37~ 38kD两条带 ,得率约为 2mg/g组织 ,对A5 49具有明显的抑制生长活性 ,抑制率 >5 0 %。结论 :此法获得的Decorin将有可能作为天然药物用于治疗肿瘤。
Purpose:The aim is to prepare and identify Decorin wh ich will be used to treat chronic inflammatory fibrosis and cancer.Method s:Decori n was extracted from rat muscle and purified by centrifugal,dialytic,chromatogra phic procedures.Results:About 37~38 kD stained band was observed on SDS-PAGE and it evidently inhibited cell growth in A549,inhibiting rate >50%. Conclusion:Decorin may have threapeutic effects against cancer.
出处
《中国生化药物杂志》
CAS
CSCD
2000年第5期237-239,共3页
Chinese Journal of Biochemical Pharmaceutics