一种新型沉淀剂去除肝素钠中蛋白质的条件研究

Study on Conditions for Eliminating Proteins from Heparin Sodium wi th a New Precipitant

目的 :探索YNB 99 1沉淀剂去除粗品肝素钠中蛋白质的作用条件。方法 :通过效价测定、蛋白质鉴定和吸收度检测 ,考察不同条件下YNB 99 1沉淀剂的沉淀效果。结果 :用 0 .0 5 %沉淀剂、5 %肝素钠溶液、效价 80USPu/mg以上的粗品和 pH3 .0的条件 ,可有效地去除蛋白质 ,肝素钠活性损失 2 %左右。 结论 :得出YNB 99 1沉淀剂的最适作用条件 ,去除蛋白质效果明显。

Purpose:To eliminate proteins from crude heparin, the reactive conditions of precipitant YNB 99-1 were investigated.Methods:The methods of potency analysis,proteins precipitate and turbidity identific ation as well as absorbance measurement at 260 nm and 280 nm were used. Results:Under the conditions of pH 3.0,5% crude heparin sodium and 0.05% Y NB 99-1,the proteins could be eliminated.Conclusion:The optimum con ditions of YNB 99-1 were obtained.Its effect was obvious.