探讨男性不育症患者解脲支原体、沙眼衣原体感染与精子质量的关系

To investigate the relationship between the infection of the ureaplasma urealyticum, chlamydia trachomatis with the sperm quality of male sterility

目的探讨男性不育症患者解脲支原体（UU）、沙眼衣原体（CT）感染与精子质量的关系。方法收集270例男性不育就诊者的精液标本，应用法国生物梅里埃公司生产的MycoplasmaIST2支原体培养，鉴定，计数及药敏试验试剂盒（比色法）、沙眼衣原体（CT）核酸检测试剂盒（PCR-荧光探针法）、精子形态染色检测（Diff-Quik法）、精子顶体酶（sAA）活性定量检测试剂盒（改良Kennedy法）、精液常规分析采用西班牙计算机精子影像图文分析系统（CASA）检测，并对uU、CT阳性纽和UU、CT阴性组的精子质量参数进行比较分析。结果UU阳性39．26％（106／270）、CT阳性7．04％（19／270）、CT和UU皆阳性2．59％（7／270），合计感染率48．89％（132／270），UU和CT皆阴性51．11％（138／270）。UU阳性组与阴性组比较，精液量少于阴性组，差异有统计学意义（P〈0．01）；CT阳性组与阴性组比较，精液量、精子浓度、总精子数和精子顶体酶（SAA）活性少于阴性组，差异有统计学意义（P〈0．01）；CT和uu皆阳性组与阴性组比较，年龄、精子鞭打快速频率（BCF）和精子顶体酶（SAA）活性少于阴性组，差异有统计学意义（P〈0．05）；有cT感染组与单-uu阳性组比较在年龄、精液量、精子非前向运动率（NP），精子浓度、总精子数、BCF和sAA均少于单-UU阳性组，差异有统计学意义（P〈0．05）。结论男性不育症患者，解脲支原体（uU）阳性率远超过沙眼衣原体（CT），但cT感染比uu感染更加影响精子质量参数，尤其是在两者混合感染的患者中。

Objective The article was discussed the relationship between the infection of the Ureaplasma urealyticum（UU） ,Chlamydia trachomatis（CT） with the sperm quality of male sterility. Methods Semen sample from 270 case of male infertility patients were detected for UU, CT. Diagnosis of urogenital mycoplasma （culture,identification,indicative enumeration, susceptibility testing） Mycoplasma IST 2;Detection Kit for Nucleic Acid to Chlamydia trachomatis（CT） （PCR-Fluorescence Probing）; Sperm Morphology Staining Kit （Diff-Quik）; Human Spermatozoa Acrosin Activity Quantitative Assay Kit（modified Kennedy）; computer-aided sperm analysis with Spain （CASA）, the Positive group of UU, CT compared to the negative group of UU, CT. Results The positive rates of UU is 39.26% （106/270） ,CT is 7.04%（19/270） ,and CT ＆ UU is 2.59%（7/270） ,The total positive rate is 48.89%（132/ 270）. The total negative rate is 51. 11% （138/270）. There were statistical difference of sperm volume between patients with UU infection and others without pathogen infection（P〈0.01） ;There were statistical difference of sperm volume, sperm concentration,the total number of sperm and sperm acrosin activity（SAA） between patients with CT infection and others without pathogen infection（P〈0.01）;There were statistical difference of age and sperm acrosin activity（SAA） between patients with CT＆UU infection and others without pathogen infection（P〈0.05）;There were statistical difference of age, sperm volume, non-progressive （motility） （NP）, sperm concentration, the total num- ber of sperm, beat-cross frequency（BCF）and sperm acrosin activity（SAA） between patients with CT infection and a single UU infection（P〈0.05）. Conclusion Positive rate of Ureaplasma urealyticum（UU） is far more than that of Chlamydia trachomatis（CT）about the article of male sterility, especially, CT infection might affect semen quality more than UU infection,especialy the mixed infection.