人类Boule基因在减数分裂时期的生物信息学分析

Bioinformatics analysis of human boule gene in meiotic

Boule(boll)基因是DAZ基因家族的一个新成员,含有一个RNA结合域和一个DAZ重复域,是人类精子发生减数分裂过程中主要调控因子。为了研究Boule基因结构及其功能,利用生物信息学方法对Boule蛋白相关结构、相互作用蛋白及其功能进行分析和预测。结果表明,Boule蛋白存在明显的亲水区、疏水区和卷曲螺旋;不存在信号肽、跨膜结构;主要分布在细胞质、细胞核、线粒体中;二级结构以α-螺旋、延伸链、无规则卷曲所组成,并含有非正规二级结构区;作用蛋白主要为CDC25A蛋白,功能域主要包含RRM保守域。Boule蛋白在精子发生过程中第一次减数分裂的生殖细胞中特异性表达,而在减数分裂完全阻滞的睾丸组织中不表达。因此,Boule蛋白功能可能与雄性生殖细胞减数分裂相关基因表达有关。

Boule （boll） gene is a new member of the DAZ gene family, which contains an RNA-binding domain and a DAZ repeat domain, and is a major regulatory factor in human spermatogenesis during meiosis. In order to study the Boule gene structure and its function, we did bioinformatics analysis and prediction of the Boule gene structure, proteins interactions and protein functions. The results show that boule proteins, have hydrophilic regions, a hydrophobic regions and coiled-coils, but any signal peptides or transmembrane; are mainly distributed in the cytoplasm, nucleus, mitochondria; are consist of e^-helix, extended strand, ruleless coiled-coil, and particularly a non-regular secondary structure area. The role of functional domains in CDC25A protein mainly comprise RRM conserved domains. Boule protein specifically expresses on germ cell in the first meiotic during spermatogenesis, and has no expression in completely block in meiosis testieular tissue. Therefore, Boule protein function may be associated with male germ cell meiosis-related gene expression.