新生儿ABO溶血病实验诊断的方法学探讨

Diagnosis of neonatal ABO hemolytic disease of experimental methodology to explore

目的探讨新生儿ABO溶血病的实验诊断方法,并筛选出更为直接、简单易行、准确度高、灵敏度好的实验诊断方法。方法收集临床疑似病例标本44例,分别用微柱凝胶法和流式细胞仪法进行新生儿溶血病三项试验(即游离试验、释放试验和直抗试验)检测。结果 44例标本中,临床最终确诊42例。微柱凝胶法检测阳性35例,诊断符合率为83.3%;流式细胞仪法检测阳性42例,诊断符合率为100%。差异有统计学意义。(P<0.05)。结论对于新生儿ABO溶血病的实验诊断,流式细胞仪法更为直接,简便易行,灵敏度高,准确度高,结果客观,易于保存,是理想的新生儿ABO溶血病临床实验室检测方法。

Objective Study of ABO hemolytic disease of the newborn screening laboratory diagnostic method,and a more direct,simple,high accuracy,good sensitivity experimental diagnostic methods. Methods Collection of specimens from 44 patients with clinically suspected cases, respectively, with micro column gel method and flow cytometric method for hemolytic disease of the newborn in three trials （i. e. , free testing, release test and detection in direct antiglobulin test ）. Results Specimens from 44 patients,clinical final diagnosis in 42 cases. The microcolumn gel assay was positive in 35 cases,diagnosis coincidence rate is 83.3%;flow cytometry assay was positive in 42 cases, diagnosis coincidence rate is 100%. The difference was statistically significant. （P〈0.05）. Conclusion For ABO hemolytic disease of the newborn of laboratory diagnosis, flow cytometry methed is more direct, simple, high sensitivity, high accuracy, objective result, easy preservation,is the ideal ABO hemolytic disease of the newborn clinical laboratory testing method.