腺病毒载体与慢病毒载体感染骨髓间充质干细胞的比较

Gene Transfer into Mesenchymal Stem Cells: a Comparison Between the Adenoviral and Lentiviral Vector Delivery Systems

随着基因治疗的发展,建立高效稳定表达目的基因的载体和靶细胞成为目前研究的热点.骨髓间充质干细胞具有自我复制,高度的增殖能力,多向或定向分化的潜能等优势,是细胞和基因工程中的理想靶细胞.而腺病毒载体和慢病毒载体作为基因载体均能将目的基因导入宿主细胞,因此对腺病毒载体和慢病毒载体的结构、分类、优缺点以及感染骨髓间充质干细胞的转染效率等方面做简要的分析.

With the increasing prominence of gene therapy, to build up stable gene delivery vectors with high expression efficiency has been considered as an attractive project. Mesenchyrnal stem cells （MSCs）, which have a high capacity for self-renewal, are muhipotent stromal cells that can differentiate into a variety of cell types. Therefore MSCs are an ideal carrier to deliver genes into the target tissues or organs. Since both adenoviral and lentiviral vectors have been used for clinical gene transfer, it is important to know the rationale behind their design, understand their classification and compare their transfection and expression efficacy.