转录组测序技术在整合网络调控中的应用现状及前景

The Application and Perspective of Transcriptome Sequencing Approach Upon Integrated Regulatory Network

2005年以来,以454焦磷酸测序技术、Solexa边合成边测序技术和SOLiD连续连接合成测序技术为标志的新一代测序技术相继诞生,使得全转录组商业化测序成为可能.针对转录因子与基因、转录因子与miRNA、以及miRNA与基因所构成的整合调控网络,转录组测序技术以高通量、高分辨率、高精度和价格低廉等优势发挥着巨大的作用.从miRNA与蛋白编码基因等层次分别介绍了转录组测序技术在基础研究、临床研究等领域的应用现状及发展前景.

Since 2005, next-generation sequencing approaches have been emerged such as the 454 se-quencing by pyrophosphoric acid, the Solexa sequencing by synthesis and the SOLID sequencing by ligation. Then the integral transcriptomes sequencing have been commercialized. The regulatory network, which com-poses the transcript factors, genes and relevant miRNA, becomes the candidate objective of next-generation sequencing. Transcriptome sequencing has enormous effect due to high-flux, high resolution, and cheap cost. The application and perspective of transcriptome sequencing approaches from two layers, e. g. miRNAs, and genes, in basal research, clinical research are reviewed.