骨碎补提取物对骨髓间充质干细胞向成骨细胞分化Cbfα-1表达的影响及意义

Effect of rhizoma drynariae extract on Cbf alpha 1 expression during the differentiation of osteoblasts by bone marrow mesenchymal stem cells and its significance

目的体外实验研究中药骨碎补对大鼠骨髓间充质干细胞增殖分化及对Cbfα-1表达的影响。方法采用中药干预培养细胞的方法,利用骨碎补单味药物与培养基联合培养大鼠骨髓间充质干细胞(BMSCs),通过Ⅰ型胶原免疫组化染色检测其促BMSCs成骨分化能力,通过RT-PCR方法进行半定量分析Cbfα-1的表达,探讨中药骨碎补促成骨能力的机制。结果骨碎补药物各组Ⅰ型胶原免疫组化染色检测BMSCs成骨分化能力及RT-PCR检测Cbfα-1的表达均明显优于空白组及条件培养组(P<0.01),0.002 g/ml药物组优于0.02 g/ml及0.0002 g/ml药物组(P<0.05)。结论中药骨碎补可促进BMSCs增殖及成骨分化,使Cbfα1的表达加强。

Objective To investigate the effect of traditional Chinese medicine （TCM）, rhizoma drynariae, on the proliferation and differentiation of rat bone marrow mesenchymal stem cells （BMSCs） and the expression of Cbf alphal （Cbfa-1） in vitro. Methods The method of culturing cells with the intervention of TCM was used. Rat BMSCs were cultured with the combination of single drug rhizoma drynariae and the medium. Collagen type I immunohistochemical staining was performed to detect its ability to promote the osteogenesis of BMSCs. The expression of Cbfa-1 was semi-quantitatively analyzed using RT-PCR, in order to investigate the mechanism of rhizoma drynariae promoting osteogenesis. Results The ability to promote the osteogenesis of BMSCs detected using collagen type I immunohistochemical staining and the expression of Cbfa-1 detected using RT-PCR in all rhizoma drynariae drug groups were significantly better than that in blank control group and conditioned culture group （P 〈 0. 01 ）. The effect in the group with 0. 002 g/ml of the drug was better than that in the groups with 0.02 g/ml and 0. 0002 g/ml of the drug （ P 〈 0.05 ）. Conclusion Rhizoma drynariae can promote the proliferation and osteogenesis differentiation of BMSCs and enhance the expression of Cbfa-l.