熊果酸对胃癌细胞株SGC-7901和MGC-803增殖及凋亡的影响

Effects of ursolic acid on proliferation and apoptosis of gastric cancer cell lines SGC-7901 and MGC-803

目的探讨熊果酸对胃癌细胞株SGC-7901和MGC-803增殖、凋亡的调控作用。方法SGC-7901和MGC-803细胞接种于含10%胎牛血清的RPMI 1640培养液中,常规培养24h后加熊果酸(10、20、40、80μmol/L),继续培养12、24、48h。MTT比色分析细胞增殖;药物作用24h后,流式细胞术检测细胞凋亡率;Western blot检测增殖细胞核抗原(PCNA)和前体半胱天冬氨酸蛋白酶3(pro-Caspase-3)的表达。结果熊果酸呈时间和剂量依赖性抑制SGC-7901和MGC-803细胞增殖;熊果酸剂量依赖性诱导SGC-7901和MGC-803细胞凋亡,抑制PCNA和pro-Caspase-3蛋白表达。结论熊果酸抑制SGC-7901和MGC-803细胞增殖并诱导其凋亡。其作用可能与抑制PCNA表达和诱导pro-Caspase-3活化有关。

Objective To investigate the regulatory effect of ursolic acid on the proliferation and apoptosis of gastric cancer cell lines of SGC-7901 and MGC-803. Methods SGC-7901 and MGC- 803 cells were seeded in RPMI-1640 supplemented with 10% heat-inactivated fetal calf serum and routinely incubated for 24 h. After serum-free starvation for 24 h, the cells were cultured with ursolic acid at the final concentration of 10, 20, 40, and 80 μmol/L for 12,24 and 48 h, respectively. Cell proliferation was determined by methyl thiazolyl tetrazolium（MTT） colorimetric assay. After treated with ursolic acid for 24 h, apoptosis rates were detected by flow cytometry. The expressions of proliferating cell nuclear antigen（PCNA） and pro-Caspase-3 were assessed by WeStern blot. Results Ursolic acid significantly inhibited SGC-7901 and MGC-803 cell proliferation in the dose- and time- dependent manners. Ursolic acid dose-dependently inhibited the expressions of PCNA and pro- Caspase-3 and induced the apoptosis rates of SGC-7901 and MGC-803 cells. Conclusion Ursolic acid can significantly inhibit cell proliferation and induce apoptosis of SGC-7901 and MGC-803 cells, which may be related to down-regulation of PCNA expression and induction of pro-Caspase-3 activation.