胰腺星状细胞对胰腺癌细胞迁移能力的影响

Effects of pancreatic stellate cells on migration of pancreatic cancer cells

目的探讨胰腺星状细胞(PSCs)对胰腺癌细胞PANC-1迁移能力的影响。方法从小鼠胰腺组织中分离PSCs后分为A组(共培养PSCs及PANC-1细胞)和B组(PANC-1细胞),均以Transwell小室培养24h。细胞划痕及细胞迁移实验分别检测两组PANC-1细胞的迁徙距离和跨膜细胞数目,免疫荧光技术及Western blot测定两组PANC-1细胞中基质金属蛋白酶(MMP)-2和MMP-9的表达。结果培养24h后,A组细胞迁移距离远于B组[(18.75±1.70)mm vs.(12.40±1.14)mm](P<0.05),跨膜细胞数目多于B组[(137±24)个vs.(68±11)个](P<0.05),MMP-2和MMP-9蛋白表达量均高于B组(0.84±0.08vs.0.41±0.13和0.56±0.72vs.0.31±0.12)(P<0.05)。结论 PSCs能够增强PANC-1细胞迁移能力,促进PANC-1细胞中MMP-2、MMP-9的表达。

Objective To explore the effects of pancreatic stellate cells（PSCs） on the migration of pancreatic cancer cells PANC-1. Methods PSCs deriveded from pancreatic tissue of mice were cultured for 24 h,which were assigned into two groups of A（PSCs and PANC1 co-cultured） and B （cultured with PANC-1 alone）. Cell migration distance and transmembrane cell number of PANC-1 were detected by wound healing assay and migration assay, respectively. The expressions of matrix metalloproteinase（MMP）-2 and MMP-9 were detected by immunofluorescence technique and Western blot. Results After cultured for 24 h,cell migration distance of PANC-1 was farther in group A than that in group BE（18. 75±1.70） mm vs. （12.40±1.14） mini （P〈0. 05）, transmembrane cell number of PANC-1 was more in group A than that in group B [（137 ± 24） pieces vs. （68 ±11） pieces] （P〈0. 05） ,and the expressions of MMP-2 and MMP-9 were higher in group A than those in group B （0.84±0.08 vs. 0.41±0.13 and 0.56±0.72 vs. 0.31±0.12）（P〈0.05）,Conelusion PSCscan enhance the ability of PANC-1 to migrate, and promote the expressions of MMP-2 and MMP-9 in PANC-1.