辣椒CaArcA基因的电子克隆与生物信息学分析

In silico Cloning and Bioinformatics Analysis of CaArcA in Capsicum annuum

目的:获得辣椒(Capsicum annuum)G蛋白β亚基基因(CaArcA)序列。方法:以拟南芥同源序列为探针,利用电子克隆技术获得了CaArcA基因的cDNA全长,采用生物信息学方法,对该基因所编码的蛋白从理化性质、分子进化、高级结构等多角度进行分析。结果:该基因cDNA全长1 173 bp,其开放阅读框为993 bp,编码330个氨基酸,分子量为36.1 kDa;具有23个磷酸化位点,无信号肽和跨膜区域,定位在细胞膜外。通过同源比对发现,CaArcA蛋白与与拟南芥(Arabidopsis thaliana)的同源蛋白相似性在80%以上。结论:利用电子克隆的方法获得一个辣椒G蛋白β亚基基因的cDNA序列。

Objective： A homologous gene of G protein beta - subunit - like wotein was obtained in Capsicum annuum. Method ： The amino acid sequence of G protein beta - subunit - like protein in Arabidopsis thaliana was used as a probe, and the homologous in Capsicum ann- uum was obtained by in silicon cloning. In addition, the bioinformatics characters were analyzed using the methods of bioinformatics in the following aspects, including the general physical and chemical properties, hydrophobicity, three dimensional structure, subcellular localiza- tion and phylogenetic relationships. Result：The cDNA length of the gene was 1 173 bp long and contained a complete ORF （993 bp） which encoded 330 amino acids. The deduced protein comprised 23 phosphorylation sites, no signal peptide and transmembrane helix. The ArcA of Capsicum annuum was a hydrophilic and chloroplast protein. The amino acid sequence of the gene showed high similarity with the homologous in other plants, such as Arabidopsis thaliana, Glycine max,Nicotiana tabacum. Conclusion ：The homologous gene of G protein beta -subunit -like protein was obtained in Capsicum annuum, and laid a solid foundation for the gene function analysis.