吉富罗非鱼感染无乳链球菌后肝脏组织在不同时期蛋白质组的表达差异

Proteome analysis of GIFT tilapia(Oreochromis niloticus) liver in different periods after Streptococcus agalactiae infection

为研究罗非鱼感染无乳链球菌前后肝脏组织蛋白质的表达变化。本研究以吉富罗非鱼为材料，采用双向电泳技术分析其在无乳链球菌感染胁迫下24h、48～144h、12d-9对照组（未感染）肝脏组织蛋白质组的变化，对差异表达蛋白进行质谱分析鉴定。结果显示：吉富罗非鱼在无乳链球菌胁迫下，3个实验组的蛋白质图谱-9对照组相比存在显著差异，共有30个蛋白质点发生显著改变，其中13个表达上调，15个下调，2个蛋白质点在感染12d后消失。通过MALDI—TOF／TOF4800质谱分析和数据库检索对这些蛋白质进行了功能分类，发现它们涉及到能量代谢、细胞防御与应激、消化免疫、抗氧化-9排毒等许多方面。推测这些蛋白质可能在吉富罗非鱼对无乳链球菌胁迫的抗性反应中发挥了重要作用。研究结果为无乳链球菌疫苗的研制及吉富罗非鱼抗病品种选育奠定了基础。

The protein expression of tilapia liver in different periods was studied after Streptococcus agalactiae infection. Two-dimensional electrophoresis was employed to analyze the proteome from liver of GIFT tilapia at 0 h,24 h,48 - 144 h and 12 days after Streptococcus agalactiae infection. The differential expressing proteins were identified by mass spectrometry. The results show that there were significant differences between experimental groups and control group in the proteomic map. Among the 30 differentially expressed proteins, 13 show an up -regulated expression and 15 shows down- regulated expression. Two proteins disappeared in the infection group 12 days later. The function classification of these proteins was analyzed by MALDI-TOF/TOF 4800 and mascot soft ware. They included association with energy metabolism proteins, cell defense and stress proteins, digestive immune proteins, immune and detoxification and other proteins. It is likely that they play an important role in the resistance responses to Streptococcus agalactiae. The discovery and identification of these proteins can offer a valuable insight into Tilapia Streptococcus lactis vaccine development and disease resistance breeding.