喉癌切缘组织中PTEN和Survivin表达与局部复发的关系

Relationship of local recurrence with the expression of PTEN and Survivin in laryngeal carcinoma and its surgical margins

目的探讨喉癌切缘组织中PTEN、Survivin表达与局部复发的关系。方法应用免疫组化SP法检测42例喉鳞状细胞癌原发灶和对应喉癌切缘组织中PTEN、Survivin蛋白的表达,术后对42例患者进行随访。结果 42例原发灶组织中PTEN、Survivin的阳性率分别为50.0%、69.0%;对应42例手术切缘组织中PTEN、Survivin的阳性率分别为83.3%、47.6%。随访期间,发生局部复发20例,复发率为47.6%。喉癌手术切缘组织中Survivin阳性者的局部复发率(75.0%)明显高于阴性者(22.7%)(P<0.05),而PTEN阳性者的局部复发率(37.1%)明显低于阴性者(100.0%)(P<0.05)。Logistic回归分析提示切缘组织中PTEN阴性、Survivin阳性,与其局部复发密切相关。结论喉癌切缘组织中PTEN表达缺失和Survivin阳性者局部复发率高,二者表达可作为预测喉癌局部复发的独立风险指标。

Purpose To explore the relationship between the expression of PTEN and Survivin proteins in the surgical margins of laryn- geal cancer and the local recurrence of tumor. Methods SP two-step immunohistochemical method was used to detect the expression of PTEN and Survivin proteins in the primary lesions and the surgical margins of laryngeal carcinoma of 42 patients, with follow-up after operation. Results The expression of PTEN and Survivin proteins in the primary lesions was 50.0% and 69.0% , and that in the sur-gical margins of laryngeal carcinoma was 83.3% and 47.6% , respectively. During follow-up, local recurrence occurred in 20 cases, and the recurrence rate was 47.6%. The recurrent rates of surgical margins positive for Survivin were 75.0%, which was significantly higher than those negative 22.7% （P 〈 0. 05 ）. The recurrent rates of surgical margins positive for PTEN were 37.1%, which was sig-nificantly lower than those negative 100.0% （ P 〈 0. 05 ）. Logistic analysis showed that the expression of PTEN and Survivin proteins in the surgical margins of laryngeal carcinoma was positively associated with the recurrent rates. Conclusion Laryngeal cancer pa-tients with Survivin-positive or PTEN-negative margin would have a higher recurrent rate. PTEN and Survivin proteins can be used as biomarkers for local recurrence of laryngeal carcinoma after operation.