槲皮素与贯叶连翘提取物合用抗抑郁作用初步研究

Synergic antidepressive effect of quercetin and Hypericum perforatum extract in mice

目的:比较不同剂量的槲皮素和贯叶连翘提取物(有效成分为贯叶金丝桃素hyperforin,HF)合用的抗抑郁效果,探索二者的协同作用。方法:雄性ICR小鼠,分为9组,即:空白对照组,阳性对照(帕罗西汀10 mg/kg)组,槲皮素组(A:5 mg/kg,B:10 mg/kg,C:20 mg/kg),贯叶连翘提取物(HF 10 mg/kg)组,合用组(A:2.5 mg/kg槲皮素+HF 5 mg/kg;B:5 mg/kg槲皮素+HF 5 mg/kg;C:10 mg/kg槲皮素+HF 5 mg/kg);灌胃给药。用利血平拮抗试验比较各组(n=11~12)拮抗利血平所致低体温、运动不能和低体温的作用,用小鼠悬尾实验比较各组(n=15)动物的悬尾不动时间。结果:合用B组的利血平拮抗作用和缩短悬尾不动时间的作用与合用C组无显著差异(P>0.05),其拮抗利血平所致低体温的作用极显著(P<0.01)强于槲皮素B组,其缩短小鼠悬尾不动时间的作用显著(P<0.05)或极显著(P<0.01)强于贯叶连翘提取物(HF 10 mg/kg)组或槲皮素B组。结论:槲皮素和贯叶连翘提取物在特定剂量比例合用时有协同抗抑郁作用。

Objective： To explore the synergistic antidepressant effect of quercetin and hyperforin （ HF, extracted from Hypericum perforatum）. Methods： Male ICR mice were divided into nine groups ： blank control, positive control （ Paroxetine, 10 mg/kg）, quercetin groups （ A：5 mg/kg, B ： 10 mg/kg, C ： 20 mg/kg）,Hypericum pe^foratum extract （HF 10 mg/kg）,combination groups （A：quercetin 2.5 mg/kg ＋ HF 5 mg/kg,B：quercetin 5 mg/kg ＋ HF 5 mg/kg,C：quercetin 10 mg/kg ＋ HF 5 mg/kg）. All drugs were administered intragastrically. Reserpine reversal tests were used to compare the reversal effects of drugs on body temperature decline, eyelid ptosis and akinesia. Tail suspension test was used to compare immobility time in each group. Results： Combination group B showed no significant difference （P 〉 0.05 ） compared with combination group C in reserpine reversal tests and tail suspension test. However, its body temperature reversal effect was significantly higher （ P 〈 0.01 ） than that of quercetin group B, and its effect in shortening immobility time was stronger than that of HF 10 mg/kg group （P 〈 0.05 ） and quercetin group B （ P 〈 0.01 ）. Conclusion： The combination of quercetin and Hypericum extract in certain ratio has significant synergistic antidepressant effect in ICR mice.