硫化氢与大鼠急性重症胰腺炎相关性的实验研究

Effect of hydrogen sulfide on severe acute pancreatitis in rats

目的探讨硫化氢(H2 S)对大鼠急性重症胰腺炎(SAP)组织损伤的影响。方法健康雄性大鼠160只,大鼠随机分四组:对照组,n=40;SAP组,n=40;SAP+硫氢化钠(NaHS)组,n=40;SAP+DL-炔丙基甘氨酸(PAG)组,n=40。每组随机分为四小组,分别对应末次注射L-Arg后3 h、12 h、24 h、36 h四个时间点,每小组n=10。分别测定血清H2 S、淀粉酶、胰腺组织中胱硫醚-γ-裂解酶(Cystathionine-γ-synthase,CSE)含量、组织病理形态学变化。结果 SAP+NaHS组血清淀粉酶较SAP组有显著升高;CSE表达与SAP组无显著性差异;镜下见比SAP组更早出现坏死和小叶结构破坏。SAP+PAG组与SAP组对照发现,预先腹腔注射PAG后,血清淀粉酶、H2 S含量及组织CSE含量明显降低,其差异有显著性,病理结果显示,显著延缓了胰腺组织损伤的出现,出血、炎症细胞浸润明显减少,Grewal胰腺病理损伤评估证实与SAP组有显著差异。结论血清H2 S含量与组织损伤呈正相关,PAG有减轻大鼠胰腺炎模型胰腺组织的损伤作用。

Objective To investigate the effect of hydrogen sulfide on severe acute pancreatitis （SAP） in rats. Methods Wistar male rats were randomly divided into 4 groups： control group （n = 40）, SAP model group （ n = 40）, SAP ＋ NariS group （ n = 40 ） and SAP ＋ PAG group （ n = 40 ）. The acute pancreatitis model was induced by in injection of L-Arg in rats and according to the time after final injection, each group were further divided into 4 subgroups（ n = 10） ： 3,12,24 h and 36 h. The H2S and amylase levels in serum and cystathionine-γ-synthase（CSE） content in pancreatic tissue were measured;the histopathological changes of pancreatic tissue were examined. Results In the SAP ＋ NariS group, the serum amylase level was significantly elevated compared to SAP group; however there was no significant difference in CSE expression between NariS group and SAP group. Microscopically, the pancreatic tissue damage was more serious and the emerging of necrosis and lobular structural failure in SAP ＋ NariS was earlier than those in SAP group. Compared to SAP group, serum amylase, H2S content and tissue CSE content SAP ＋ PAG group were reduced significantly; pathological examination showed that the pancreatic tissue damage was significantly delayed, the bleeding and inflammatory cells infiltration significantly reduced and Grewal pancreas pathological damage assessment also demonstrated Conclusion Serum H2S level is positively correlated a significant difference with the SAP group. with tissue damage in severe acute pancreatitis of rats, and PAG can relieve the tissue damage of acute pancreatitls.