咖啡因可保护UVB致人皮肤成纤维细胞的损伤

Caffeine can protect human fibroblasts damage from ultraviolet-B irradiation

目的:确定咖啡因对中波紫外线(UVB)照射致体外培养人皮肤成纤维细胞氧化损伤的防护作用。方法:分离培养人成纤维细胞,分为空白对照组、咖啡因组、UVB照射组、UVB照射+咖啡因组,UVB照射剂量为30 mJ/cm^2。用MTT法检测细胞增殖活性,酶生化比色法检测细胞丙二醛(MDA)含量、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活性。结果:UVB照射前后用咖啡因处理能使成纤维细胞存活率提高、MDA产生减少,酶的活性增强。结论:咖啡因对UVB照射损伤成纤维细胞具有一定保护性作用,其机制可能与抑制氧化损伤和增强抗氧化能力有关。

Objective： To determine the protective effect of caffeine on UVB-indueed skin damage. Meth- ods： Cultured human dermal fibroblast was separated, and divided into： blank group, caffeine group, UVB ir- radiation group and the group of UVB irradiation plus caffeine. The cells were irradiated with UVB of 30 mJ/ cm2. Cellular activity was detected by M3T assay. The level of MDA, superoxide dismutase （SOD） and gluta- thione peroxidase （GSH-Px） was detected by enzyme biochemical assay. Results： Caffeine inhibited lipid peroxidation, decreased MDA content and increased the activity of SOD and GSH-Px after UVB irradiation. Conclusion： Caffeine has a certain protective effect on UVB irradiation damage of Human Fibroblasts, which may be related to the inhibition of oxidative damage and enhanced antioxidant capacity.