薯蓣皂苷元通过MAPK通路对食管癌细胞Eca109的调控

Diosgenin regulates proliferation,apoptosis,migration and invasion of human esophageal cancer Eca109 cells via the MAPK signaling pathway

目的:探讨薯蓣皂苷元(diosgenin,Dio)对人食管癌Eca109细胞的增殖、凋亡、迁移和侵袭的影响以及其作用机制.方法:MTT和Transwell实验检测薯蓣皂苷元对肿瘤细胞的增殖、凋亡、迁移和侵袭的影响.Western blot检测薯蓣皂苷元处理后的Eca109细胞丝裂原活化蛋白激酶(mitogenactivated protein kinase,MAPK)信号通路中c-jun氨基末端应激活化蛋白激酶(c-Jun N-terminal of stress-activated protein kinase,J N K),细胞外信号调节激酶(e x t r a c e l l u l a r signal-regulated kinase,Erk1/2)和p38丝裂原激活蛋白激酶(p38 mitogen-activated protein kinase,p38)的蛋白表达水平.结果:食管癌Eca109细胞经过50 g/mL的薯蓣皂苷元处理48 h后,与未经药物处理的对照组相比,细胞的增殖、迁移(16.54 vs 34.12,P<0.05)和侵袭(9.42 vs 26.99,P<0.05)效应显著下降,细胞的凋亡效应明显增加(0.24 vs0.64,P<0.05).Eca109细胞中p-p38蛋白的表达水平明显降低(1.66vs 0.23,P<0.05),而JNK、Erk1/2、p38、p-JNK、p-Erk1/2蛋白表达水平的差异并不显著.结论:薯蓣皂苷元可能通过p-p38途径调控人食管癌细胞的增殖、凋亡、迁移和侵袭.

AIM： To investigate the effects of diosgenin on the proliferation, apoptosis, migration and inva-sion of human esophageal Eca109 cells and to explore the underlying mechanisms. METHODS： MTT and transwell experiments were executed to detect the effects of diosgenin on the proliferation, migration and invasion of Eca109 cells. Western blot was applied to detect the expression of c-Jun N-terminal of stress-acti-vated protein kinase （JNK）, extracellular signal-regulated kinase （Erk1/2） and the p38 kinase of the mitogen-activated protein kinase （MAPK） pathway. RESULTS： Compared with the control group, Eca109 cells treated with 50 μg/mL of diosgenin for 48 h showed significantly decreased prolif-eration, migration （16.54 vs 34.12, P 〈 0.05） and invasion （9.42 vs 26.99, P 〈 0.05） but increased apoptosis （0.24 vs 0.64, P 〈 0.05）. Moreover, di-osgenin treatment significantly decreased the expression of p-p38 protein （1.66 vs 0.23, P 〈 0.05） but had no significant effect on the expression of JNK, Erkl/2, p38, p-JNK, or p-Erk1/2. CONCLUSION： Diosgenin regulates the pro-liferation, apoptosis, migration and invasion of Eca109 cells possibly via the p-p38 pathway.