ZIC1和P130在胆管细胞癌中表达及其临床意义

Clinical significance of expression of ZIC1 and P130 in cholangiocarcinoma

目的:探讨锌指蛋白1(zicfamilymember1,ZIC1)和P130蛋白在胆管细胞癌组织中的表达及其与胆管细胞癌生物学行为的关系.方法:应用免疫组织化学SP法检测43例胆管细胞癌和20例正常胆管上皮组织中ZIC1和P130蛋白的表达情况.结果:胆管细胞癌组织中ZIC1的表达显著高于正常胆管组织(53.49%vs 25.00%,P<0.05),P130表达显著低于正常胆管组织(55.81%vs 85.00%,P<0.05);ZIC1和P130蛋白在胆管细胞癌中的表达与病理分级和是否转移相关(P<0.05),与患者性别,Nevin分期不相关(P>0.05);ZIC1和P130蛋白表达呈明显负相关(r=-0.360,P<0.05);ZIC1和P130蛋白在胆管细胞癌中的表达与3年生存率有明显差异(P<0.05).结论:ZIC1和P130蛋白的异常表达在胆管细胞癌发生发展中具有重要意义,多个指标联合检查用来估计患者预后指导意义更佳.

AIM： To investigate the expression of zicfami-lymemberl （ZIC1） and P130 in cholangiocarci- noma and their correlation with the biological behavior of cholangiocarcinoma. METHODS： The protein expression of ZIC1 and P130 was detected by immunohistochemistry in 43 cases of cholangiocarcinoma and 20 cases of normal bile duct tissue. RESULTS： The positive rate of ZIC1 expres-sion was significantly higher in cholangiocarci-noma than in normal bile duct tissue （53.49% vs 25.00%, P 〈 0.05）. The positive rate of P130 ex-pression in cholangiocarcinoma was significant-ly lower than that in normal bile duct tissue （55.81vs 85.00%, P 〈 0.05）. The expression of ZIC1 and P130 protein was related to pathological grade and neoplasm metastasis （both P 〈 0.05）, but not to sex or Nevin stage （both P 〉 0.05）. There was a negative correlation between ZIC1 and P130 expression （r = -0.360, P 〈 0.05） in cholangiocar-cinoma. The expression of ZIC1 and P130 pro-teins was related to the 3-year survival rate （both P 〈 0.05）. CONCLUSION： The abnormal expression of ZIC1 and P130 proteins may be involved in the development and progression of cholangiocarci-noma.