益生菌对大鼠非酒精性脂肪肝的治疗作用及机制

Effect of probiotics treatment on rat non-alcoholic fatty liver disease

目的:观察益生菌制剂对高脂饮食诱导的非酒精性脂肪肝(nonalcoholic fatty liver disease,NAFLD)大鼠的防治作用.方法:以高脂饲料喂养制备大鼠肥胖并发脂肪肝模型.8 wk后将造模成功大鼠分为治疗组和脂肪肝对照组.治疗组给予双歧杆菌三联活菌肠溶胶囊5g/(kg·d)灌胃;脂肪肝对照组给予相同体积的生理盐水灌胃.于16 wk末处死大鼠,称量肝脏及脂肪质量,计算肝指数,肝脏组织制作石蜡切片行HE染色,观察病理变化;检测各组大鼠血清中血脂、肝功能、脂肪因子等指标.结果:模型组大鼠与对照组相比,血脂、肝功能水平及肝指数增高,血清中白介素6(interleukin-6,IL-6)、肿瘤坏死因子-(tumor necrosis factor-,TNF-)、胰岛幕抵抗指数(homeostasis model assessment of insulin resistance,HOMA-IR)水平显著升高,光镜下肝脏出现明显脂肪变性及炎细胞浸润;益生菌干预后的大鼠,与模型组大鼠比较,肝指数、肝功能、血脂降低,光镜下的肝脏脂肪变性及炎细胞浸润明显改善,血清中脂联素水平明显上升,IL-6、TNF-、HOMA-IR水平显著下降.结论:益生菌制剂可显著减轻NAFLD大鼠肝脏脂肪变性,改善肝功能,对NAFLD有显著治疗效果.

AIM： To assess the effect of probiotics treatment on rat non-alcoholic fatty liver disease （NALFD）. METHODS： SD rats were fed a high-fat diet to in- duce NAFLD. After 8 weeks, the model rats were randomly divided into a saline-treated group and a therapy group [bifidobacterium triple viable enteric-coated pills 5 g/（kg.d）]. At the end of 16 weeks, all rats were killed. Weight of the body and the liver, the indexes of serum lipid, liver en- zyme and adenosine were measured. Pathologi- cal changes such as inflammatory cell infiltration and steatosis in the liver were observed. RESULTS： Compared to the normal control group, serum levels of glutamic-oxalacetic trans- aminase （AST）, glutamic-pyruvic transaminase （ALT）, total cholesterol （TC）, triglyceride （TG）, liver mass index, interleukin-6 （IL-6）, tumor ne- crosis factor-~ （TNF-~）, and homeostasis model assessment of insulin resistance （HOMA-IR） in the NAFLD model group markedly increased, and obvious steatosis and inflammatory cell infiltration were noted. After treatment with probiotics, TG, AST, ALT, liver mass index, ste- atosis and inflammatory cell infiltration were improved significantly, and the level of adipo- nectin was increased. CONCLUSION： Probiotics can remarkably al- leviate dyslipidemia and liver steatosis in a rat model of NAFLD.