Id2在Apc^(Δ716/+)小鼠肠道肿瘤发生中的作用

The role of Id2 in the growth of intestinal tumor in Apc^(Δ716/+) mice SHEN Xiaodong

目的研究DNA结合抑制物2(inhibitor of DNA binding 2,Id2)在ApcΔ716/+小鼠肠道肿瘤发生中的作用。方法 Id2基因缺失的基因工程小鼠和肠道肿瘤模型ApcΔ716/+小鼠杂交后,统计ApcΔ716/+小鼠与ApcΔ716/+Id2-/-杂交小鼠小肠肠道肿瘤的数目及总负荷,观查其肠道肿瘤发生和发展的变化。结果与ApcΔ716/+Id2野生型小鼠比较,ApcΔ716/+Id2-/-杂交小鼠在4、8、12周龄时肠道肿瘤总数目与不同大小的肠道肿瘤数目明显减少,差异有统计学意义(P均<0.05)。结论 Id2的缺失能抑制ApcΔ716/+小鼠肠道肿瘤的发生,Id2在人类肠道肿瘤中可能发挥着促进肿瘤形成的作用。

Objective To investigate the role of Id2 in the development and growth of intestinal tumor in ApcΔ716/＋ mice. Methods ApcΔ716/＋ mice were crossed with Id2 deficient mice and the tumor susceptibility in intestine was com- pared. The total intestine tumor number and the tumor number of different size of ApcΔ716/＋ Id2 ＋/＋ mice and ApcΔ716/＋ Id2 /- mice were analyzed and the changes in intestinal tumor were observed. Results Compared with ld2 wild in ApcΔ716/＋ mice, Id2 deficient in ApcΔ716/＋ mice resulted in significant decrease of tumor total number and tumor number of different size in intestine at 4, 8 and 12 weeks, respectively （P 〈 0.05 ）. Conclusion These findings have provided the genetic evidence for a tumor promotion role of Id2 in the intestine of mice. Importantly, these studies also suggest that Id2 may act as a tumor promotor for human intestinal tumors.