绝经后妇女甲状旁腺素基因多态性与骨密度:福州地区的调查

The association between polymorphism of parathyroid hormone gene and bone mineral density in Fuzhou postmenopausal women

背景:有研究证实,绝经后妇女骨密度与甲状旁腺素基因有密切关系,但在不同地区人群中结果存在差异性。目的:探讨福州地区绝经后妇女甲状旁腺素基因(PTH)BstBⅠ多态性与骨密度的关系。方法:用双能X射线骨密度仪检测福州地区150例绝经后妇女的腰椎、股骨颈,大转子和Ward’s三角骨密度,应用PCR-RFLP技术检测甲状旁腺素基因BstBⅠ多态性。结果与结论:①甲状旁腺素基因型分布频率为BB型68.8%、Bb型24.1%、bb型7.1%。等位基因频率为B81%,b 19%,基因型分布符合Hardy-Weinberg定律。②分析其基因型与骨密度的关系:BB、Bb、bb 3种基因型在股骨颈、大转子、Ward’s三角区4个部位骨密度差异均无显著意义(P>0.05)。甲状旁腺素基因BstBⅠ位点多态性与骨密度间无关联,尚不能作为预测福州地区绝经后妇女发生骨质疏松危险的遗传标志。

BACKGROUND： Studies have shown the bone mineral density of postmenopausal women is closely related to parathyroid hormone. But there are differences in different areas. OBJECTIVE： To investigate the association betweenBstB I polymorphism of parathyroid hormone gene with bone mineral density in postmenopausal women from Fuzhou area. METHODS： The bone mineral densities of the lumbar spine, femoral neck, trochanter and Ward＇s triangle were measured in 150 postmenopausal women by dual energy X-ray absorptiometry. The genotype of parathyroid hormone gene was determined by polymerase chain reaction-restriction fragment length polymorphism. RESULTS AND CONCLUSION： （1） The distribution of parathyroid hormone genotypes were BB genotype 68.8%, Bb 24.1%, and bb 7.1%. The B allelic gene frequencies reached 81%, while b was 19%. The distribution followed the Hardy-Weinberg equilibrium. （2） Analysis of the relationship between the genotypes and bone mineral density： There was no significant difference in the bone mineral densities of the lumbar spine, femur, neck, trochanter and Ward＇s triangle among the three genotypes （P 〉 0.05）. BstB I gene polymorphism of parathyroid hormone gene is not correlated to bone mineral density, and there is no enough evidence to support genotype of parathyroid hormone gene as a genetic marker in predicting the risk of developing osteoperosis in Fuzhou postmenopausal women.