增生性瘢痕中时序变化的骨形态发生蛋白及其受体

Expression of bone morphogenetic protein and its receptors in different stages of hypertrophic scar

背景:骨形态发生蛋白与瘢痕的形成和发展相关,而成纤维细胞与瘢痕的增殖、成熟关系密切,但骨形态发生蛋白在不同时期增生性瘢痕成纤维细胞中的表达变化尚少见报道。目的:检测骨形态发生蛋白2/4、骨形态发生蛋白7、骨形态发生蛋白IA型受体在不同时期增生性瘢痕成纤维细胞中的表达水平。方法:应用免疫组织化SP法检测来自2008年3月至2010年7月广西壮族自治区人民医院美容整形外科患者的增殖期增生性瘢痕和成熟期增生性瘢痕中成纤维细胞中骨形态发生蛋白2/4、骨形态发生蛋白7、骨形态发生蛋白IA型受体的表达。结果与结论:增生性瘢痕增殖期成纤维细胞中骨形态发生蛋白2/4的表达水平显著高于成熟期(P<0.05);但不同时期增生性瘢痕成纤维细胞中骨形态发生蛋白7和骨形态发生蛋白IA型受体的表达差异无显著性意义(P>0.05)。表明在增生性瘢痕由增生期至成熟期的发展过程中,其成纤维细胞中的骨形态发生蛋白2/4的表达下调,而骨形态发生蛋白7、骨形态发生蛋白IA型受体的表达无变化。

BACKGROUND： Bone morphogenetic proteins are associated with the formation and development of scars. Fibroblasts are closely related to the proliferation and maturation of scars. However, the expression of bone morphogenetic proteins in fibroblasts in different stage of hypertrophic scars remains poorly understood. OBJECTIVE： To observe the expression of bone morphogenetic protein 2/4, bone morphogenetic protein 7, bone morphogenetic protein receptor IA in fibroblasts in different stage of hypertrophic scars. METHODS： The immunohistochemical SP method was employed to detect the expression of bone morphogenetic protein 2/4, bone morphogenetic protein 7, bone morphogenetic protein receptor IA in fibroblasts in 20 cases of proliferative stage hypertrophic scar and 20 cases of maturation stage hypertrophic scar. All samples were obtained from the Department of Plastic and Aesthetic Surgery, the People＇s Hospital of Guangxi Zhuang Autonomous Region, China. RESULTS AND CONCLUSION： The expression of bone morphogenetic protein 2/4 in fibroblasts in proliferative hypertrophic scars significantly elevated compared with mature hypertrophic scars （P 〈 0.05）. In various stages of hypertrophic scars, the expression of bone morphogenetic protein 7 and bone morphogenetic protein receptor IA in fibroblasts was not obviously different （P 〉 0.05）. Results demonstrated that bone morphogenetic protein 2/4 expression down-regulated during the development from proliferative stage to maturation stages in fibroblasts in hypertrophic scars. However, there was no change in the expression of bone morphogenetic protein 7 and bone morphogenetic protein receptor IA.