热量限制提高氧化损伤的SH-SY5Y细胞的超氧化物歧化酶活性

Caloric restriction increases activity of superoxide dismutase in oxidatively damaged SH-SY5Y cells

目的观察热量限制培养条件下,氧化损伤的SH-SY5Y细胞超氧化物歧化酶活性。方法体外培养SH-SY5Y细胞,分为对照组(3.0 g/L L-glucose)、H2O2损伤组(250μmol/L H2O2)、低糖组(2.0 g/L L-glucose)、低糖+损伤组、白藜芦醇(1.25μmol/L)组、白藜芦醇(1.25μmol/L)+损伤组,测定各组细胞的超氧化物歧化酶活性。结果用低糖和白藜芦醇1.25μmol预处理细胞24 h,给予250μmol/L H2O2损伤细胞1 h,超氧化物岐化酶活性测定结果显示,与对照组比较,损伤组SOD减少,低糖组明显增多,白藜芦醇1.25μmol/L组无明显改变;与损伤组比较,低糖+损伤组明显增加,白藜芦醇+损伤组稍有增加。给予250μmol/L H2O2损伤细胞7 h,与对照组比较,损伤组SOD减少,低糖组明显增多,白藜芦醇1.25μmol/L组无明显改变;与损伤组比较,低糖+损伤组明显增加,白藜芦醇+损伤组稍有增加。低糖+损伤组的SOD活性明显高于白藜芦醇+损伤组。结论低糖培养可提高细胞培养基中超氧化物歧化酶的活性,提示热量限制可能通过增加SH-SY5Y细胞的抗氧化系统酶的活性,减少氧化产物的含量,降低氧化应激的水平,保护细胞免受氧化损伤的攻击,延缓相关疾病的发生发展。白藜芦醇不通过提高细胞培养基中超氧化物歧化酶活性发挥抗氧化损伤作用。

Objective To detect and clarify the superoxide dismutase （SOD） activity in oxidatively damaged human neuroblastoma SH-SYSY cells cultured under conditions of caloric restriction. Methods SH-SYSY cells cultured in vitro, were divided into six groups： control group （3.0 g/L glucose）, H2O2 damage （250 μmol/L） group, low glucose （2.0 g/L glucose） group, low glucose ＋ H2O2 damage group, resveratrol （1.25 μmol/L） group, and resveratrol （1.25 μmol/L） ＋ H2O2（250 μmol/L） damage group. The SOD activity of all groups was determined. Results After the pretreatment with low glucose and resveratrol （ 1.25 μmol/L） for 24 hours and treatment with 250 μmol/L H2O2 for 1 h, compared with that of the control group, the SOD activity was significantly decreased in the H2O2 damage group, significantly increased in the low glucose group, and not significantly changed in the resveratrol （1.25 μmol/L） group. Compared with the H2O2 damage group, the SOD activity was significantly increased in the low glucose ＋ H2O2 group, and slightly increased in the resveratrol ＋ H2O2 damage group. After treated with 250 μmol/L H2O2 for 7 hours, compared with the control group, the SOD activity was decreased in the H2O2 damage group, significantly increased in the low glucose group, and not significantly changed in the resveratrol （1.25 μmol/L） group. Compared with the H2O2 damage group, the SOD activity was significantly increased in the low glucose ＋ H2O2 damage group, and slightly increased in the resveratrol ＋ H2O2 damage group. The SOD activity in the low glucose ＋ H2O2 damage group was significantly higher than that in the resveratrol ＋ H2O2 damage group. Conclusions Low sugar treatment can improve the SOD activity in cultured cells, suggesting that caloric restriction by increasing the antioxidant enzymes activity in SH-SY5Y cells, reducing oxidation products, decreasing the level of oxidative stress, protects the cells from oxidative damage attacks and slows the occurrence and development of related diseases. Resveratrol does not improve the anti-oxidative damage in the cells through increasing the SOD activity in the culture medium.