小鼠经寰枢椎蛛网膜下腔置管法的建立

Chronic catheterization of the atlantoaxial spinal subarachnoid space in mice

目的介绍一种新型小鼠蛛网膜下腔置管法,并与传统的Wu方法相比较,为脊髓水平的药物生理研究及疼痛治疗提供更有效方法。方法选择40只24-28 g雄性昆明小鼠,随机分为Wu组和寰枢椎（AA）组,分别从腰5-6椎间隙、寰枢椎间隙置管至蛛网膜下腔。模型建立后,观察各组小鼠置管后死亡率、肢体瘫痪率,并测定其缩足反应阈值（50%PWT）、甩尾反应潜伏期（TF）和吗啡作用。对比两组是否有差异。结果置管后Wu组和AA组在死亡率无差异的情况下,AA组的肢体瘫痪率明显低于Wu组;术后7 d内,AA组大鼠的50%PWT值和TF值与术前基础值相比最为接近;吗啡镇痛及耐受实验说明此新方法可以进行小鼠长期给药。结论小鼠经寰枢椎蛛网膜下腔置管方法具有瘫痪率低、恢复快、术后并发症少等优点,是一种新型有效的小鼠鞘内置管法。

Objective To establish a new method of catheterization of the spinal subarachnoid space in mice and to compare with the traditional catheterization, which is used for researches of neurophysiology and pain treatment. Methods Forty male Kunming mice weighing 24-28 g were randomly divided into two groups： the group Wu and atlantoaxial group （group AA）. The group AA received catheterization of the atlantoaxial spinal subarachnoid space, and the group Wu received catheterization in the lumbar 5 - 6 spinal subarachnoid space. After the models were established, we observed the death rate and limb paralysis, measured the 50% paw withdrawal threshold （50% PWT） , tail flick latency and analgesic effect of morphine to compare the differences between the two groups. Results The death rate was the same in the groups Wu and AA. The percentage of limb paralysis was obviously lower in the group AA than in the group Wu. Within 7 days of catheterization, the 50% PWT and the TF of AA group were closest to the baseline. Morphine analgesia experiment showed that the new method is suitable for long-term injection. Conclusions The mouse model of atlantoaxial catheterization of spinal subarachnoid space is an effective intrathecal catheterization method, with aolvantages such as faster recovery and less injury.