川贝母对大鼠油酸型急性呼吸窘迫综合征的影响

Therapeutic effect of tendrilleaf fritillary bulb on acute respiratory distress syndrome rats

目的:观察川贝母对油酸所致急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)大鼠肺组织的影响。方法:通过静脉注射油酸法建立大鼠ARDS模型。将40只模型大鼠随机均分为ARDS模型组,川贝母低剂量组,川贝母高剂量组,地塞米松组。另取10只Wistar大鼠作为空白对照组。在造模后0.5 h首次给药,1次/d,连续3 d。川贝母高、低剂量组分别给予5.0,2.5 mg/kg川贝母生药灌胃,空白对照组与模型组给予生理盐水灌胃,1 mL/100 g,地塞米松组给予地塞米松灌胃,10 mg/kg。测定各组大鼠左肺湿/干重比值(W/D),检测肺组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量;酶联免疫吸附法测定血清肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)的含量。结果:与空白对照组相比,模型组大鼠左肺W/D、MDA、TNF-α、IL-1β、IL-6含量显著升高,SOD活性降低(P均<0.05);与模型组相比,川贝母高、低剂量组及地塞米松组大鼠左肺W/D、MDA、TNF-α、IL-1β、IL-6含量显著降低,SOD活性升高(P均<0.05)。结论:川贝母可减轻油酸所致ARDS大鼠的肺组织病理损害程度,其机制可能是通过阻止体内脂质过氧化过程而实现。

Objective： To observe the effect of tendrilleaf fritillary bulb on acute respiratory distress syndrome（ARDS） rats induced by oleic acid.Methods： The ARDS model was constructed by injection of oleic acid intravenously.Forty model rats were randomly divided into ARDS model group,low-dose tendrilleaf fritillary bulb group,high-dose tendrilleaf fritillary bulb group and dexamethasone group.Another ten Wistar rats were selected as the control group.Left lung wet / dry weight（W / D） ratio,lung tissue superoxide dismutase（SOD） activity,malondialdehyde（MDA） content were detected,serum levels of tumor necrosis factor-α（TNF-α）,interleukin-1β（IL-1β）,interleukin-6（IL-6） levels were assayed by enzyme linked immunosorbent assay.Results： Compared with the control group,the model group showed lower levels of lung wet / dry weight（W / D） ratio,MDA,TNF-α,IL-1β,IL-6,but with higher levels of SOD activity（P 0.01 or P 0.05）.The tendrilleaf fritillary bulb groups showed lower levels of lung wet / dry weight（W/D） ratio,MDA,TNF-α,IL-1β,IL-6,but higher levels of SOD,compared with the model group（both P 0.05）.Conclusion： Tendrilleaf fritillary bulb could reduce lung tissue damage induced by oleic acid,and has the therapeutic effect on ARDS rat,which may be by preventing lipid peroxidation in the course of ARDS.