肺癌p53基因表达和突变的研究

The Study of p53 Gene Expression and Mutation in Lung Cancer

目的 :探讨肺癌p53基因表达和突变的临床意义。方法 :采用流式细胞术、聚合酶链反应及单链构象多态技术对 2 3例肺癌p53基因表达和突变进行了研究。结果 :p53基因表达的阳性率为4 3 5% ,p53基因突变率为 39 2 % ;p53基因表达与突变基本一致且倾向分布于中低分化的病例 ,而与临床分期及组织学类型的关系不明显。结论 :p53基因的异常在肺癌的发生过程中可能属早期事件 。

Objective:To study the clinical significance of p53 gene expression and mutation in lung cancer.Methods:23 cases of lung cancer were examined for p53 gene expression & mutation by means of flow cytometry, polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP).Results:p53 positive expression was detected in 43 5% of the cases and the percentage of p53 gene mutation was 39 2%. The expression and mutation were basically consistent and tend to occur in the cases of low and middle differentiation. The expression and mutation didn't relate with clinic stage and histologicl types. Conclusion:p53 gene alteration may be the early event in the progression of lung cancer and helpful to determine the degree of lung cancer differentiation.