不同佐剂和激发方式对哮喘小鼠肺部炎性反应的影响

The effect of different adjuvants and challenge methods on asthma mice′s lung inflammation

目的研究不同佐剂和激发方式对哮喘小鼠的肺部炎性反应发生和发展的影响。方法应用无特定病原体(SPF)级的BALB/c小鼠,以卵清蛋白作过敏原,采用氢氧化铝和十二水硫酸铝钾作佐剂,雾化7d后比较炎性反应表现,选用合适的佐剂后用滴鼻和雾化的激发方式制备小鼠哮喘模型,对肺泡灌洗液(BALF)的白细胞进行计数及分类和观察肺组织中气管和血管周围的炎性反应。结果采用十二水硫酸铝钾激发哮喘BALF中白细胞和嗜酸性粒细胞比例比氢氧化铝高(P<0.05),肺组织炎性反应更明显。5次及7次雾化均比3次滴鼻激发哮喘的BALF中白细胞和嗜酸性粒细胞比例高(P<0.05),肺组织炎性反应更明显;5次与7次雾化激发哮喘,炎性反应无明显差别;停止雾化10d后炎性反应明显减轻。结论在建立哮喘模型中,十二水硫酸铝钾比氢氧化铝能更明显地诱导肺部炎性反应;雾化比滴鼻更能诱导肺部炎性反应;结束雾化一段时间后炎性反应会消退。

Objective To investigate the effect of different adjuvants and challengemethods on asthma mice′s lung inflammation . Methods BALB/c mice were sensitized with ovalbumin in aluminium hydroxide or alum adjuvant .After 7 days challenges ,choose the proper adjuvant with intranasal or atomized to prepare mice asthma model .Count and classify the white blood cells （WBC） in bronchoalveolar lavage fluid（BALF） ,observe the peribronchial and perivascular inflammation .Results Compare the WBC and eos-nophils in BALF ,the alum group are higher than aluminum hydroxide group（P〈0 .05） ,5 and 7 days′atomized groups are highter than 3 days′intranasal group（P〈0 .05） ,5 and 7 days′atomized groups have showed no significant difference （P〉0 .05） .10 days after the last challenge ,the inflammation fade sharply ,and the corresponding result occured in the lung inflammation .Conclusion In establishing asthma model ,alum is better than aluminum hydroxide ,atomization is better than intranasal .Inflammation will fade after stop atomization a period of time .