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Different Techniques of Screening Differentially Expressed Genes and Their Applying in Tea Plant

Different Techniques of Screening Differentially Expressed Genes and Their Applying in Tea Plant
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摘要 The genome of common varieties of tea plant,with estimated size 4000 Mb,is not clearly yet.In order to understand the gene expression difference during certain biological process,we can use transcriptome which reflects the gene expression level and is more closely related to current status instead of genome.This paper reviews several widely used techniques to screen differentially expressed genes in transcriptome level by comparing the characteristics of the methods and their application in tea plant research. The genome of common varieties of tea plant,with estimated size 4000 Mb,is not clearly yet.In order to understand the gene expression difference during certain biological process,we can use transcriptome which reflects the gene expression level and is more closely related to current status instead of genome.This paper reviews several widely used techniques to screen differentially expressed genes in transcriptome level by comparing the characteristics of the methods and their application in tea plant research.
出处 《茶叶》 2013年第4期223-230,共8页 Journal of Tea
基金 financially supported by the National Science Foundation of China (Project No.31170643)
关键词 差异表达基因 茶树 筛选 技术 基因表达差异 转录水平 生物过程 基因组 Differentially expressed genes tea plant DDRT-PCR cDNA-AFLP SSH cDNA microarray DGE RNA-Seq
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