6-BA和KT对干旱条件下小麦旗叶甘油醛-3-磷酸脱氢酶及光合作用的影响

Effect of 6-BA and KT on glyceraldehy-3-phospate dehydrogenase activity and phytosynthesis in wheat flag leaves under soil drought

以干旱敏感和抗旱品种冀麦 38和晋麦 33为试验材料 ,采用花期控水使土壤自然干旱的胁迫 (土壤相对含水量每天约下降 10 %左右 )方法 ,研究了不同抗旱性小麦旗叶中的甘油醛 - 3 -磷酸脱氢酶 (GAPD)活性、气孔阻力和光合速率等指标变化 ,结果表明 :干旱胁迫下 ,不同抗旱性小麦旗叶GAPD活性随着土壤相对含水量(SRWC)的下降而逐渐降低 ,在小麦扬花期叶面喷施 10 -5mol·L-1的 6 -苄氨基腺嘌呤 (6 -BA)或激动素 (KT) ,均有助于提高小麦旗叶GAPD的活性 ,当SRWC为 46 .6 %时 ,经 6 -BA或KT处理的冀麦 38旗叶的GAPD活性分别为 1.82nmolNADPH·mg-1protein·min-1和 1.75nmolNADPH·mg-1protein·min-1,分别比干旱胁迫喷水高 35 .8%和 30 .6 %。同时 ,6 -BA和KT均能对干旱胁迫下不同抗旱性小麦旗叶的光合速率和气孔阻力有一定的影响。 6

In this paper two wheat (Triticum aestivum L.)cultivars with different drought resistance(drought resistant Jinmai 33 and drought sensitive Jimai 38) were used as experimental materials.The effect of water stress induced by soil drought on the glyceraldehyde-3-phosphate dehydrogenase(GAPD),the stomatal resistance and net phytosynthetic rate were studied.The results indicated that with reduction of soil relative water content(SRWC) in two difference wheat flag leaves,the activity of GAPD was decreased.While spraying 10 -5 mol·L -1 6-benzylamin opurine (6-BA) or kinetin (KT) on flag leaf at the anthesis stage both increased the activity of GAPD in two cultivars.And during drought stress stage,6-BA or KT both effected the stomatal resistance and the net phytosynthetic rate of the wheat flag leaves.The effect oftreatment of 6-BA was better than that of KT.