姜黄素对人肝癌细胞株 He pG2巨噬细胞迁移抑制因子表达的影响

Effect of Curcumin on the Expression of Macrophage Migration Inhibitory Factor in Human Hepatocellular Carcinoma Cell Line HepG2

[目的]检测人肝癌细胞株HepG2中巨噬细胞迁移抑制因子（MIF）的表达及释放，探讨姜黄素对HepG2中MIF表达的影响。[方法]反转录聚合酶链反应（RT-PCR）及免疫印迹法（Western Blot）检测对照组及不同浓度姜黄素干预组（10，20，30，40，50μmol/L）HepG2中MIF mRNA及蛋白表达水平；ELISA检测对照组及不同浓度姜黄素干预组（10，20，30，40，50μmol/L ）培基中 M IF蛋白水平。[结果]HepG2高表达MIF mRNA及MIF蛋白，各浓度姜黄素干预均可抑制HepG2中MIF mRNA及MIF蛋白的表达，且呈浓度依赖效应（ P ＜0．05）；HepG2可自分泌MIF蛋白，各浓度姜黄素干预均可抑制 HepG2中MIF 蛋白的释放，且呈浓度依赖效应（ P ＜0．05）。[结论]人肝癌细胞株HepG2可表达及释放MIF ；姜黄素在转录和翻译水平能有效抑制M IF的表达及释放，这可能是其发挥抗肿瘤效应的分子机制之一。

[Objective] To detect the expression and releasing of macrophage migration inhibitory factor （MIF） in human hepatocellular carcinoma cell line HepG2 .and to explore the effect of curcumin on the expres-sion of MIF in HepG2 .[Methods] Reverse transcription polymerase chain reaction （RT-PCR） and Western blotting method were used to detect the expression of MIF mRNA and protein in control group and curcumin treatment groups of different concentrations（10 ,20 ,30 ,40 and 50μmol/L） .The expression of MIF protein in control group and curcumin treatment groups of different concentrations （10 ,20 ,30 ,40 and 50μmol/L） was measured by using ELISA .[Results] The expression of MIF mRNA and protein in HepG2 was high .Curcu-min with different concentrations could inhibit the expression of MIF mRNA and protein in HepG 2 in dose-de-pendent manner（ P＆lt;0 .05） .MIF protein could be released by HepG2 .Curcumin with different concentrations could inhibit the releasing of MIF protein in HepG2 in dose-dependent manner（ P＆lt;0 .05） .[Conclusion]MIF is expressed in HepG2 and released by HepG2 .Curcumin can effectively inhibit the expression and releasing of MIF at transcription and translation level ,which may be one of the mechanisms of anti-neoplastic effect of cur-cumin .