土茯苓中鞣质提取工艺的优化

Optimization on Extraction Technology of Tannin fromSmilax Glabra Roxb.

为了提高土茯苓的应用价值,对土茯苓中鞣质的提取工艺进行了探究。以丙酮溶液作为提取剂回流提取,采用络合滴定法测定鞣质的提取量,通过单因素实验和正交实验对土茯苓中鞣质的提取工艺进行优化,并采用精密度实验、重复性实验以及加标回收率实验对方法进行了评价。结果表明,最佳提取工艺为:提取温度70℃、丙酮浓度40%、料液比1∶15(g∶mL)、提取时间90min,在此条件下,土茯苓中鞣质的提取量为20.77mg·g-1。该提取工艺经济可行,方法的精密度高、重现性好。

In order to improve the application value of Smilax glabra Roxb.,extraction technology of tannin from Smilax glabra Roxb.was explored.Acetone solution was used as extractant in reflux extraction method.The eomplexometric titration method was used for determination of the tannin extract.The extraction technology of tannin from Smilax glabra Roxb.was optimized by single factor experiment and orthogonal experiment.Precision experiment,repeated experiment and standard addition recovery experiment were used to evaluate the method.The results showed that the optimum extraction conditions were as follows：extraction temperature 70 ℃,acetone concentration 40％,solid-liquid ratio 1.∶ 15（g：mL）,extraction time 90 min.The tannin extract from Smilax glabra Roxb.was 20.77 mg · g-1 under above conditions.The extraction technology is economic and feasible with high precision and good reproducibility.