乙型肝炎病毒对胰岛素样生长因子结合蛋白7表达的影响及其临床意义

The influence of hepatitis B virus on the expression of insulin-like growth factor-binding protein 7 and its clinical significance

目的探讨乙型肝炎病毒(HBV)对胰岛素样生长因子结合蛋白7(IGFBP7)表达的影响及其临床意义。方法逆转录-聚合酶链反应(PCR)检测IGFBP7 mRNA的表达水平,酶联免疫吸附试验(ELISA)检测血清IGFBP7含量,分析IGFBP7在慢性乙型肝炎(CHB)、肝纤维化(LC)和肝细胞癌(HCC)患者间血清含量的差异。结果 IGFBP7 mRNA在HepG2.2.15细胞中的表达水平较HepG2高;与健康对照组相比,HBV感染者IGFBP7血清学水平明显升高(P<0.05);IGFBP7在LC和HCC患者低于CHB患者(P<0.05)。结论 HBV能够上调IGFBP7的表达,其血清水平与疾病进程呈负相关。

Objective To investigate the influence of hepatitis B virus （HBV） on the expression of insulin-like growth factor-binding protein 7 （ IGFBP7 ） and its clinical significance. Methods The mRNA expression of IGFBP7 was measured by reverse transcription-polymerase chain reaction （ PCR ） , and serum levels of IGFBP7 were measured by enzyme-linked immunosorbent assay（ ELISA）. The different expressions of IGFBP7 among patients with chronic hepatitis B （CHB） , liver cirrhosis （LC） and hepatocellular carcinoma（HCC） were analyzed. Results The expression of IGFBP7 mRNA was higher in HepG2.2.15 cells than in HepG2 cells. Compared with healthy controls, serum IGFBP7 levels were much higher in HBV patients （ P 〈 0.05 ） , and the serum levels of IGFBP7 were detected at lower levels in patients with LC and HCC than those with CHB （ P 〈 0.05 ）. Conclusions HBV can upregulate the expression of IGFBP7, which is negatively correlated with the disease progression.