三磷酸腺苷发光法在耐多药结核分枝杆菌诊断中的运用

The application of adenosine triphosphate bioluminescence assay in the diagnosis of multidrug-resistant Mycobacterium tuberculosis

目的通过与罗氏固体培养法比较,评估三磷酸腺苷发光法检测耐多药结核分枝杆菌的可行性。方法采用三磷酸腺苷发光法与罗氏固体培养法同时检测和分析149例临床分离的结核分枝杆菌。结果三磷酸腺苷发光法与罗氏固体培养法的一致率为92.6%(138/149),差异无统计学意义(χ2=0.57,P=0.45)。三磷酸腺苷发光法检测时间为(6.6±2.1)d,明显快于传统罗氏固体培养法的28 d(t=422.7,P<0.001)。结论与常规检测方法比较,三磷酸腺苷发光法具有快速、简便、准确性高等优点,对耐多药结核患者的早期诊断和耐药结核分枝杆菌流行的控制有很大帮助,适合实验室开展。

Objective To compare with Roche solid culture method, and to evaluate the feasibility of adenosine triphosphate bioluminescence assay for detecting multidrug-resistant Mycobacterium tuberculosis. Methods By Roche solid culture method and adenosine triphosphate bioluminescence assay, 149 clinical isolates of Mycobacterium tuber culosis were determined and analyzed. Results The coincidence rate of adenosine triphosphate bioluminescence assay with Roche solid culture method was 92.6% （138/149）, and the difference had no statistical significance （X^2 = 0.57, P = 0.45）. The detection time of adenosine triphosphate bioluminescence assay was （ 6.6 ±2.1 ） d, which was faster than that of Roche solid culture method （ 28 d, t = 422.7, P 〈 0. 001 ）. Conclusions Compared with the conventional detection methods, adenosine triphosphate bioluminescence assay is simple, rapid and accurate. It is helpful for detecting muhidrug-resistant tuberculosis patients and controlling the prevalence of Mycobacterium tuberculosis. It is suitable for clinical laboratory.