人感染H7N9禽流感监测病例中分离到一株A型呼吸道合胞病毒

A strain of RSV-A isolated from the monitoring specimens of avian influenza A (H7N9) virus

目的对H7N9型禽流感病毒(简称H7N9病毒)监测病例进行病原学检测,以明确呼吸道感染的诊断与鉴别诊断。方法采用多重逆转录聚合酶链反应(RT-PCR)对H7N9病毒进行监测的同时对22种呼吸道常见病原体核酸进行检测,并对检测到的病原体进行分离鉴定。结果在一例重症肺炎患者的H7N9病毒监测标本中检出A型呼吸道合胞病毒(RSV-A),并利用人胚肺成纤维细胞(MRC-5)对该标本进行了病毒分离,成功分离到了一株RSV-A。结论多重RT-PCR能同时对22种呼吸道病原核酸进行检测,有助于疫情的及时诊断和控制。RSV-A的成功分离为呼吸道合胞病毒的进一步研究打下基础。

Objective To detect and diagnose respiratory pathogens in the monitoring specimens of avian influenza A （H7Ng） virus. Methods Nucleic acids of 22 kinds of respiratory pathogens were determined by muhiple reverse transcription polymerase chain reaction （RT-PCR）. Relative respiratory pathogens were isolated and indentified. Results Among the monitoring specimens of avian influenza A （ HTN9 ） virus, nucleic acid of respiratory syncytial virus A （RSV-A） was determined from a severe pneumonia illness patient. The specimen was isolated by MRC-5, and 1 strain of RSV-A was isolated successfully. Conclusions Multiple RT-PCR could test 22 kinds of respiratory pathogens simultaneously and contributes to diagnose and control the epidemic situation. The isolation of RSV-A would provide a foothold for the further research of respiratory syncytial virus.